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PMID: 12586708 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of Ras-induced overproliferation in Drosophila hemocytes.

Genetics ·Vol. 163 ·No. 1 ·2003-01-00 ·Pages 203-15

Asha H, Nagy I, Kovacs G, Stetson D, Ando I, Dearolf CR

Abstract

We use the Drosophila melanogaster larval hematopoietic system as an in vivo model for the genetic and functional genomic analysis of oncogenic cell overproliferation. Ras regulates cell proliferation and differentiation in multicellular eukaryotes. To further elucidate the role of activated Ras in cell overproliferation, we generated a collagen promoter-Gal4 strain to overexpress Ras(V12) in Drosophila hemocytes. Activated Ras causes a dramatic increase in the number of circulating larval hemocytes (blood cells), which is caused by cellular overproliferation. This phenotype is mediated by the Raf/MAPK pathway. The mutant hemocytes retain the ability to phagocytose bacteria as well as to differentiate into lamellocytes. Microarray analysis of hemocytes overexpressing Ras(V12) vs. Ras(+) identified 279 transcripts that are differentially expressed threefold or more in hemocytes expressing activated Ras. This work demonstrates that it will be feasible to combine genetic and functional genomic approaches in the Drosophila hematopoietic system to systematically identify oncogene-specific downstream targets.

MeSH Terms
Animals Cell Division/genetics,physiology Drosophila/genetics,metabolism Hemocytes/metabolism Oligonucleotide Array Sequence Analysis Promoter Regions, Genetic ras Proteins/genetics,metabolism
Chemicals
ras Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Asha H
Department of Pediatrics, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
Nagy Istvan
Kovacs Gabor
Stetson Daniel
Ando Istvan
Dearolf Charles R
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/163.1.203
Published
2003-01-00
Pages
203-15
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1462399
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/163.1.203 PMC Full Text View journal details
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