Abstract
Polarization of the C. elegans zygote along the anterior-posterior axis depends on cortically enriched (PAR) and cytoplasmic (MEX-5/6) proteins, which function together to localize determinants (e.g. PIE-1) in response to a polarizing cue associated with the sperm asters. Using time-lapse microscopy and GFP fusions, we have analyzed the localization dynamics of PAR-2, PAR-6, MEX-5, MEX-6 and PIE-1 in wild-type and mutant embryos. These studies reveal that polarization involves two genetically and temporally distinct phases. During the first phase (establishment), the sperm asters at one end of the embryo exclude the PAR-3/PAR-6/PKC3 complex from the nearby cortex, allowing the ring finger protein PAR-2 to accumulate in an expanding 'posterior' domain. Onset of the establishment phase involves the non-muscle myosin NMY-2 and the 14-3-3 protein PAR-5. The kinase PAR-1 and the CCCH finger proteins MEX-5 and MEX-6 also function during the establishment phase in a feedback loop to regulate growth of the posterior domain. The second phase begins after pronuclear meeting, when the sperm asters begin to invade the anterior. During this phase (maintenance), PAR-2 maintains anterior-posterior polarity by excluding the PAR-3/PAR-6/PKC3 complex from the posterior. These findings provide a model for how PAR and MEX proteins convert a transient asymmetry into a stably polarized axis.
MeSH Terms
14-3-3 Proteins
Animals
Caenorhabditis elegans/embryology
Caenorhabditis elegans Proteins/genetics,metabolism
Green Fluorescent Proteins
Helminth Proteins/genetics,metabolism
Luminescent Proteins/genetics,metabolism
Mutation
Nuclear Proteins/genetics,metabolism
Proteins/genetics,metabolism
Receptor, PAR-2
Receptors, Thrombin/genetics,metabolism
Recombinant Fusion Proteins/genetics,metabolism
Tyrosine 3-Monooxygenase/genetics,metabolism
Zygote/physiology
Chemicals
14-3-3 Proteins
Caenorhabditis elegans Proteins
Helminth Proteins
Luminescent Proteins
MEX-5 protein, C elegans
MEX-6 protein, C elegans
Nuclear Proteins
Proteins
Receptor, PAR-2
Receptors, Thrombin
Recombinant Fusion Proteins
par-5 protein, C elegans
par-6 protein, C elegans
pie-1 protein, C elegans
Green Fluorescent Proteins
Tyrosine 3-Monooxygenase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cuenca Adrian A
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Schetter Aaron
Aceto Donato
Kemphues Kenneth
Seydoux Geraldine
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