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PMID: 1260 Published · ppublish English Comparative Study Journal Article

Axoplasm chemical composition in Myxicola and solubility properties of its structural proteins.

The Journal of physiology ·Vol. 253 ·No. 1 ·1975-12-00 ·Pages 303-19

Gilbert DS

Abstract

The chemical composition of axoplasm extracted from the giant axon of Myxicola infundibulum has been analysed, and some of the factors which disperse its gel structure have been identified. 2. The axoplasm contains about 3-6% protein, and 0-12% lipid. It is isosmotic with sea water and has a pH near 7-0. 3. Inorganic ions in extracted axoplasm include: Na+, 13m-mole/kg wet wtl; K+, 280; Cl-, 24; Ca2+, 0-3; Mg2+, 3. 4. Free organic ions in axoplasm include: gly, 180 m-mole/kg wet st.; cysteic acid, 120; asp, 75; glu, 10; ala, 7; tau, 5; thr, 2; gln and ser, trace; homarine, 63; isethionate, 0. 5. The gel structure is dispersed by solutions containing 1--10 mM-Ca2+, because this ion activates an endogenous protease. The gel can also be dispersed without proteilysis by solutions containing 0-5 M-KCl, or 0-5 M guanidine hydrochloride, or 3-5 M urea, all of which break down neurofilaments. 6. It is argued that many aspects of the composition and dispersal properties of Myxicola axoplasm are similar to those in other axons.

MeSH Terms
Amino Acids/analysis Animals Axons/analysis Calcium/analysis Chlorides/analysis Cysteic Acid/analysis Cytoplasm/analysis Glycine/analysis Hydrogen-Ion Concentration Lipids/analysis Magnesium/analysis Nerve Tissue Proteins/analysis Osmolar Concentration Picolinic Acids/analysis Polychaeta/analysis Potassium/analysis Sodium/analysis
Chemicals
Amino Acids Chlorides Lipids Nerve Tissue Proteins Picolinic Acids Sodium Cysteic Acid Magnesium Potassium Calcium Glycine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Gilbert D S
References (43)
43 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1975.sp011191
Published
1975-12-00
Pages
303-19
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1348544
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1975.sp011191 PMC Full Text View journal details
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