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PMID: 1262786 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of DNA excreted from phytohemagglutinin-stimulated lymphocytes.

The Journal of experimental medicine ·Vol. 143 ·No. 5 ·1976-05-01 ·Pages 1249-64

Rogers JC

Abstract

The DNA released into the culture medium after phytohemagglutinin (PHA) stimulation of human peripheral blood lymphocytes has been purified and characterized. It is double stranded, sediments at 7-8S in alkaline sucrose, and has a Tm determined optically and by thermal elution from hydroxyapatite that is substantially lower than that found for lymphocyte cell DNA. Media DNA contains a major component reassociating with an average Cot-1/2 of 87 mol X s/liter, compared to a Cot-1/2 of 770 mol X s/liter for the unique fraction of cell DNA as measured by reassociation in 0.6 M Na+. This component of media DNA consists of unique sequence elements which are largely shared in media DNA preparations from cultures derived from different cell donors. The marked difference between media DNA and cell DNA indicates that media DNA is not derived from cell death and lysis, rather than some unique portion of lymphocyte DNA is apparently excreted from the cells during PHA-stimulated growth.

MeSH Terms
Chromatography DNA/analysis,metabolism Humans Kinetics Lectins/pharmacology Lymphocyte Activation Lymphocytes/analysis,immunology Molecular Weight Nucleic Acid Denaturation Nucleic Acid Renaturation
Chemicals
Lectins DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Rogers J C
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22 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.143.5.1249
Published
1976-05-01
Pages
1249-64
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190176
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.143.5.1249 PMC Full Text View journal details
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