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PMID: 12644507 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

CheZ phosphatase localizes to chemoreceptor patches via CheA-short.

Journal of bacteriology ·Vol. 185 ·No. 7 ·2003-04-00 ·Pages 2354-61

Cantwell BJ, Draheim RR, Weart RB, Nguyen C, Stewart RC, Manson MD

Abstract

We have investigated the conditions required for polar localization of the CheZ phosphatase by using a CheZ-green fluorescent protein fusion protein that, when expressed from a single gene in the chromosome, restored chemotaxis to a DeltacheZ strain. Localization was observed in wild-type, DeltacheZ, DeltacheYZ, and DeltacheRB cells but not in cells with cheA, cheW, or all chemoreceptor genes except aer deleted. Cells making only CheA-short (CheA(S)) or CheA lacking the P2 domain also retained normal localization, whereas cells producing only CheA-long or CheA missing the P1 and P2 domains did not. We conclude that CheZ localization requires the truncated C-terminal portion of the P1 domain present in CheA(S). Missense mutations targeting residues 83 through 120 of CheZ also abolished localization. Two of these mutations do not disrupt chemotaxis, indicating that they specifically prevent interaction with CheA(S) while leaving other activities of CheZ intact.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,genetics,metabolism Chemoreceptor Cells/metabolism Chemotaxis/genetics Conserved Sequence Enterobacteriaceae/metabolism Green Fluorescent Proteins Hydrophobic and Hydrophilic Interactions Luminescent Proteins/genetics,metabolism Models, Molecular Molecular Sequence Data Mutation Protein Conformation Protein Kinases/chemistry,genetics,metabolism Protein Structure, Tertiary Recombinant Fusion Proteins/genetics,metabolism Sequence Homology, Amino Acid Subcellular Fractions
Chemicals
Bacterial Proteins Luminescent Proteins Recombinant Fusion Proteins Green Fluorescent Proteins Protein Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cantwell Brian J
Department of Biology, Texas A&M University, College Station, Texas 77843, USA.
Draheim Roger R
Weart Richard B
Nguyen Cameran
Stewart Richard C
Manson Michael D
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.7.2354-2361.2003
Published
2003-04-00
Pages
2354-61
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC151485
Subset
IM
Grants
NIGMS NIH HHS · GM37369 · United States
NIGMS NIH HHS · GM52583 · United States
External Links
PubMed source DOI: 10.1128/JB.185.7.2354-2361.2003 PMC Full Text View journal details
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