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PMID: 12644660 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Proteome analysis. Novel proteins identified at the peribacteroid membrane from Lotus japonicus root nodules.

Plant physiology ·Vol. 131 ·No. 3 ·2003-03-00 ·Pages 1080-90

Wienkoop S, Saalbach G

Abstract

The peribacteroid membrane (PBM) forms the structural and functional interface between the legume plant and the rhizobia. The model legume Lotus japonicus was chosen to study the proteins present at the PBM by proteome analysis. PBM was purified from root nodules by an aqueous polymer two-phase system. Extracted proteins were subjected to a global trypsin digest. The peptides were separated by nanoscale liquid chromatography and analyzed by tandem mass spectrometry. Searching the nonredundant protein database and the green plant expressed sequence tag database using the tandem mass spectrometry data identified approximately 94 proteins, a number far exceeding the number of proteins reported for the PBM hitherto. In particular, a number of membrane proteins like transporters for sugars and sulfate; endomembrane-associated proteins such as GTP-binding proteins and vesicle receptors; and proteins involved in signaling, for example, receptor kinases, calmodulin, 14-3-3 proteins, and pathogen response-related proteins, including a so-called HIR protein, were detected. Several ATPases and aquaporins were present, indicating a more complex situation than previously thought. In addition, the unexpected presence of a number of proteins known to be located in other compartments was observed. Two characteristic protein complexes obtained from native gel electrophoresis of total PBM proteins were also analyzed. Together, the results identified specific proteins at the PBM involved in important physiological processes and localized proteins known from nodule-specific expressed sequence tag databases to the PBM.

MeSH Terms
Cell Membrane/genetics,physiology Databases, Genetic Enzymes/genetics,metabolism Immunity, Innate/genetics Lotus/genetics,metabolism,microbiology Membrane Transport Proteins/genetics,metabolism Plant Proteins/genetics,metabolism Plant Roots/genetics,metabolism,microbiology Proteome/genetics,metabolism Rhizobiaceae/growth & development Signal Transduction/genetics,physiology
Chemicals
Enzymes Membrane Transport Proteins Plant Proteins Proteome pathogenesis-related proteins, plant
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wienkoop Stefanie
Department of Plant Research, Risø National Laboratory, Roskilde, Denmark.
Saalbach Gerhard
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.102.015362
Published
2003-03-00
Pages
1080-90
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC166873
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.102.015362 PMC Full Text View journal details
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