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PMID: 12665620 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Circadian phase-specific degradation of the F-box protein ZTL is mediated by the proteasome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 8 ·2003-04-15 ·Pages 4933-8

Kim WY, Geng R, Somers DE

Abstract

Critical to the maintenance of circadian rhythmicity is the cyclic expression of at least some components of the central oscillator. High-amplitude cycling of mRNA and protein abundance, protein phosphorylation and nuclear/cytoplasmic shuttling have all been implicated in the maintenance of circadian period. Here we use a newly characterized Arabidopsis suspension cell culture to establish that the rhythmic changes in the levels of the clock-associated F-box protein, ZTL, are posttranscriptionally controlled through different circadian phase-specific degradation rates. This proteolysis is proteasome dependent, implicating ZTL itself as substrate for ubiquitination. This demonstration of circadian phase-regulated degradation of an F-box protein, which itself controls circadian period, suggests a novel regulatory feedback mechanism among known circadian systems.

MeSH Terms
Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics,metabolism Cells, Cultured Circadian Rhythm Cysteine Endopeptidases/metabolism Multienzyme Complexes/metabolism Photoperiod Proteasome Endopeptidase Complex Protein Processing, Post-Translational RNA, Messenger/genetics,metabolism RNA, Plant/genetics,metabolism
Chemicals
Arabidopsis Proteins Multienzyme Complexes RNA, Messenger RNA, Plant ZTL protein, Arabidopsis Cysteine Endopeptidases Proteasome Endopeptidase Complex
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kim Woe-Yeon
Department of Plant Biology/Plant Biotechnology Center, Ohio State University, Columbus, OH 43210, USA.
Geng Ruishuang
Somers David E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0736949100
Published
2003-04-15
Epub
2003-00-28
Pages
4933-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC404699
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0736949100 PMC Full Text View journal details
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