Home LiteratureArticle Details
PMID: 12694599 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

High-Cot sequence analysis of the maize genome.

The Plant journal : for cell and molecular biology ·Vol. 34 ·No. 2 ·2003-04-00 ·Pages 249-55

Yuan Y, SanMiguel PJ, Bennetzen JL

Abstract

Higher eukaryotic genomes, including those from plants, contain large amounts of repetitive DNA that complicate genome analysis. We have developed a technique based on DNA renaturation which normalizes repetitive DNA, and thereby allows a more efficient outcome for full genome shotgun sequencing. The data indicate that sequencing the unrenatured outcome of a Cot experiment, otherwise known as High-Cot DNA, enriches genic sequences by more than fourfold in maize, from 5% for a random library to more than 20% for a High-Cot library. Using this approach, we predict that gene discovery would be greater than 95% and that the number of sequencing runs required to sequence the full gene space in maize would be at least fourfold lower than that required for full-genome shotgun sequencing.

MeSH Terms
Base Sequence Cloning, Molecular/methods Genome, Plant Genomic Library Interspersed Repetitive Sequences/genetics Molecular Sequence Data Nucleic Acid Hybridization Sequence Homology, Nucleic Acid Zea mays/genetics
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yuan Yinan
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.
SanMiguel Phillip J
Bennetzen Jeffrey L
Article Info
Journal
The Plant journal : for cell and molecular biology
Abbr.
Plant J
ISSN
0960-7412
Published
2003-04-00
Pages
249-55
Language
English
Region
England
NLM ID
9207397
Subset
IM
Databases
GENBANK
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