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PMID: 12695516 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

YB-1 as a cell cycle-regulated transcription factor facilitating cyclin A and cyclin B1 gene expression.

The Journal of biological chemistry ·Vol. 278 ·No. 30 ·2003-07-25 ·Pages 27988-96

Jurchott K, Bergmann S, Stein U, Walther W, Janz M, Manni I, Piaggio G, Fietze E, Dietel M, Royer HD

Abstract

Expression of the Y-box protein YB-1 is increased in proliferating normal and cancer cells, but its role in cell proliferation and cell cycle progression is unclear. We have identified a cell cycle-dependent relocalization of YB-1 from the cytoplasm to the nucleus at the G1/S phase transition and demonstrate that both the charged zipper and the cold shock domain are involved in regulating this process. Using cell lines that constitutively overexpress YB-1, we show that nuclear accumulation of YB-1 is associated with increased cyclin A and cyclin B1 mRNA and protein expression. We provide evidence that deregulated YB-1 expression is linked to adhesion-independent cell proliferation through the induction of cyclin A. Thus, we have identified YB-1 as a cell cycle stage-specific transcription factor important for cell proliferation.

MeSH Terms
Amino Acid Motifs Breast Neoplasms/metabolism CCAAT-Enhancer-Binding Proteins/metabolism,physiology Cell Adhesion Cell Cycle Cell Division Cell Line Cell Nucleus/metabolism Cell Separation Cyclin A/genetics,metabolism Cyclin B/genetics,metabolism Cyclin B1 Cytoplasm/metabolism DNA-Binding Proteins Flow Cytometry G1 Phase Gene Expression Regulation, Neoplastic Genes, Reporter HeLa Cells Humans Immunoblotting Immunohistochemistry Kinetics Microscopy, Fluorescence Mutation NFI Transcription Factors Nuclear Proteins Promoter Regions, Genetic Protein Structure, Tertiary RNA, Messenger/metabolism Reverse Transcriptase Polymerase Chain Reaction S Phase Subcellular Fractions/metabolism Transcription Factors/metabolism Transfection Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins CCNB1 protein, human Cyclin A Cyclin B Cyclin B1 DNA-Binding Proteins NFI Transcription Factors Nuclear Proteins RNA, Messenger Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Jurchott Karsten
Max-Delbrück-Centrum für Molekulare Medizin, Robert-Rössle-Strasse 10, 13092 Berlin, Germany. [email protected]
Bergmann Stephan
Stein Ulrike
Walther Wolfgang
Janz Martin
Manni Isabella
Piaggio Giulia
Fietze Ellen
Dietel Manfred
Royer Hans-Dieter
Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
Published
2003-07-25
Epub
2003-00-14
Pages
27988-96
Language
English
Region
United States
NLM ID
2985121R
Subset
IM
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