Home LiteratureArticle Details
PMID: 1270140 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Purification of staphylococcal alpha-toxin by adsorption chromatography on glass.

Infection and immunity ·Vol. 13 ·No. 3 ·1976-03-00 ·Pages 982-6

Cassidy P, Harshman S

Abstract

Staphylococcal alpha-toxin was purified from Staphylococcus aureus growth medium using adsorption chromatography on controlled pore glass beads. Elution of alpha-toxin from the unmodified glass surface of the beads with various anions generally followed the chaotropic series. Alpha-toxin, purified by glass bead chromatography, is composed of a single electrophoretic form, containing less than 2% of other forms.

MeSH Terms
Adsorption Animals Anions Chromatography/methods Glass Hemolysis Humans In Vitro Techniques Rabbits Staphylococcus aureus Toxins, Biological/isolation & purification
Chemicals
Anions Toxins, Biological
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cassidy P
Harshman S
References (11)
11 references, click to expand
  1. Purification and properties of two forms of staphylococcal toxin.
    Biochemistry. 1973 Jul 3;12(14):2672-7 PMID: 4197123
  2. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
    Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
  3. Application of permeation chromatography on controlled-pore glass in the purification of plant viruses.
    Acta Virol. 1972 Jun;16(1):53-62 PMID: 4400676
  4. Purification and some properties of staphylococcal alpha-toxin.
    Jpn J Exp Med. 1974 Apr;44(2):165-78 PMID: 4546168
  5. Some biological properties of purified staphylococcal haemolysins.
    Toxicon. 1972 Aug;10(5):511-9 PMID: 5071600
  6. Isolation and composition of staphylococcal alpha toxin.
    J Gen Microbiol. 1963 Mar;30:455-68 PMID: 13967637
  7. Chromatography of biological materials on polyethylene glycol-treated controlled-pore glass.
    Prep Biochem. 1972;2(2):193-203 PMID: 4112864
  8. Destabilization of membranes with chaotropic ions.
    Methods Enzymol. 1974;31:770-90 PMID: 4370616
  9. Purification of D-beta-hydroxybutyrate apodehydrogenase, a lecithin-requiring enzyme.
    Methods Enzymol. 1974;32:374-91 PMID: 4475348
  10. Physical and chemical studies on staphylococcal -toxins A and B .
    Biochemistry. 1973 Jul 3;12(14):2677-83 PMID: 4711471
  11. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
    Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.13.3.982-986.1976
Published
1976-03-00
Pages
982-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC420702
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.13.3.982-986.1976 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2