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PMID: 1271524 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Method for the isolation of bacteriphage T4 mutants that produce particles with giant capsids.

Journal of virology ·Vol. 18 ·No. 2 ·1976-05-00 ·Pages 785-7

Paietta JV, McDonald TL, Doermann AH

Abstract

An efficient procedure is described for isolating bacteriophage T4 mutants that produce particles with giant capsids. The selective parameters of the technique rely primarily on the giants' characteristic density in cesium gradients and on their resistance to UV radiation.

MeSH Terms

Capsid Centrifugation, Density Gradient Coliphages/isolation & purification,radiation effects,ultrastructure DNA Viruses Mutation Radiation Effects Ultraviolet Rays Viral Proteins

Chemicals

Viral Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Paietta J V

McDonald T L

Doermann A H

References (5)

5 references, click to expand

Genetic control of capsid length in bacteriophage T4. I. Isolation and preliminary description of four new mutants.
J Virol. 1973 Aug;12(2):374-85 PMID: 4127051
The transformation of tau particles into T4 heads. II. Transformations of the surface lattice and related observations on form determination.
J Supramol Struct. 1974;2(2-4):253-75 PMID: 4612249
Phenotypic mixing of host range and serological specificities in bacteriophages T2 and T4.
Virology. 1956 Jun;2(3):388-98 PMID: 13325403
Lysis and Lysis Inhibition with Escherichia coli Bacteriophage.
J Bacteriol. 1948 Feb;55(2):257-76 PMID: 16561455
High Negative Interference over Short Segments of the Genetic Structure of Bacteriophage T4.
Genetics. 1958 May;43(3):332-53 PMID: 17247760

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.18.2.785-787.1976

Published

1976-05-00

Pages

785-7

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC515608

Subset

External Links

PubMed source DOI: 10.1128/JVI.18.2.785-787.1976 PMC Full Text View journal details

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Method for the isolation of bacteriphage T4 mutants that produce particles with giant capsids.

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