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PMID: 12744469 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Expression of antioxidant enzymes in astrocytic brain tumors.

Brain pathology (Zurich, Switzerland) ·Vol. 13 ·No. 2 ·2003-04-00 ·Pages 155-64

Haapasalo H, Kyläniemi M, Paunul N, Kinnula VL, Soini Y

Abstract

We studied the expression of antioxidant enzymes (AOEs) and related proteins manganese superoxide dismutase (MnSOD), thioredoxin (Trx), thioredoxin reductase (TrxR), and the catalytic (GLCL-c) and regulatory (GLCL-r) subunits of glutamate cysteine ligase (gamma-glutamylcysteinesynthetase) in 433 astrocytomas. Expression of MnSOD was found in 91%, Trx in 46%, TrxR in 66%, GLCL-c 73% and GLCL-r in 89% of the cases. Diffuse astrocytomas showed more intense staining for Trx (p = 0.002), TrxR (p = 0.004), GLCL-c (p = 0.001), GLCL-r (p = 0.04) and MnSOD (p = 0.01) than pilocytic astrocytomas. Within diffuse astrocytomas only Trx (p = 0.0001) and TrxR (p= 0.04) significantly associated with increased malignancy grade. Necrotic tumors were more often immunopositive for Trx (p = 0.001) and TrxR (p = 0.02) and AOE expression was generally higher in mitotically active tumors. Expression of Trx and lack of MnSOD expression was associated with a worse prognosis in diffuse astrocytomas. None of the AOEs had any prognostic value in pilocytic grade I astrocytomas. Familial astrocytomas, which included 23 of the cases studied, did not differ in their expression of MnSOD from sporadic ones. The results show that MnSOD and Trx may influence the biological behaviour of astrocytomas, possibly by modulating cell proliferation and necrosis in these tumors.

MeSH Terms
Antioxidants/metabolism Astrocytoma/enzymology,genetics,mortality Brain Neoplasms/diagnosis,enzymology,genetics Cell Transformation, Neoplastic/genetics Free Radical Scavengers/metabolism Gene Expression Profiling Glioblastoma/enzymology,genetics,mortality Glutamate-Cysteine Ligase/genetics Humans Immunohistochemistry Oligonucleotide Array Sequence Analysis Oxidoreductases/metabolism Prognosis Superoxide Dismutase/genetics Survival Analysis Thioredoxin-Disulfide Reductase/genetics Thioredoxins/genetics Tissue Distribution
Chemicals
Antioxidants Free Radical Scavengers Thioredoxins Oxidoreductases Superoxide Dismutase Thioredoxin-Disulfide Reductase Glutamate-Cysteine Ligase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Haapasalo Hannu
Department of Pathology, Tampere University Hospital, Tampere, Finland.
Kyläniemi Maarit
Paunul Niina
Kinnula Vuokko L
Soini Ylermi
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Article Info
Journal
Brain pathology (Zurich, Switzerland)
Abbr.
Brain Pathol
ISSN
1015-6305
DOI
10.1111/j.1750-3639.2003.tb00015.x
Published
2003-04-00
Pages
155-64
Language
English
Region
Switzerland
NLM ID
9216781
PMCID
PMC8096025
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1750-3639.2003.tb00015.x PMC Full Text View journal details
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