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PMID: 12754249 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The glycosyltransferase gene encoding the enzyme catalyzing the first step of mycothiol biosynthesis (mshA).

Journal of bacteriology ·Vol. 185 ·No. 11 ·2003-06-00 ·Pages 3476-9

Newton GL, Koledin T, Gorovitz B, Rawat M, Fahey RC, Av-Gay Y

Abstract

Mycothiol is the major thiol present in most actinomycetes and is produced from the pseudodisaccharide 1D-myo-inosityl 2-acetamido-2-deoxy-alpha-D-glucopyranoside (GlcNAc-Ins). A transposon mutant of Mycobacterium smegmatis shown to be GlcNAc-Ins and mycothiol deficient was sequenced to identify a putative glycosyltransferase gene designated mshA. The ortholog in Mycobacterium tuberculosis, Rv0486, was used to complement the mutant phenotype.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism Cysteine DNA Transposable Elements Disaccharides/biosynthesis Glycopeptides Glycosyltransferases/genetics,metabolism Inositol Molecular Sequence Data Mutation Mycobacterium smegmatis/enzymology,genetics Mycobacterium tuberculosis/enzymology,genetics Pyrazoles Sequence Alignment Sulfhydryl Compounds
Chemicals
Bacterial Proteins DNA Transposable Elements Disaccharides Glycopeptides Pyrazoles Sulfhydryl Compounds mycothiol Inositol Glycosyltransferases Cysteine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Newton Gerald L
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, USA.
Koledin Teresa
Gorovitz Batia
Rawat Mamta
Fahey Robert C
Av-Gay Yossef
References (21)
21 references, click to expand
  1. Expression and characterization of the Mycobacterium tuberculosis serine/threonine protein kinase PknB.
    Infect Immun. 1999 Nov;67(11):5676-82 PMID: 10531215
  2. Characterization of Mycobacterium smegmatis mutants defective in 1-d-myo-inosityl-2-amino-2-deoxy-alpha-d-glucopyranoside and mycothiol biosynthesis.
    Biochem Biophys Res Commun. 1999 Feb 16;255(2):239-44 PMID: 10049692
  3. Alteration of a single amino acid residue reverses fosfomycin resistance of recombinant MurA from Mycobacterium tuberculosis.
    Microbiology. 1999 Nov;145 ( Pt 11):3177-84 PMID: 10589726
  4. Photoaffinity labelling with P3-(4-azidoanilido)uridine 5'-triphosphate identifies gpi3p as the UDP-GlcNAc-binding subunit of the enzyme that catalyses formation of GlcNAc-phosphatidylinositol, the first glycolipid intermediate in glycosylphosphatidylinositol synthesis.
    Biochem J. 2000 Sep 15;350 Pt 3:815-22 PMID: 10970797
  5. N-Acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) is a key enzyme in mycothiol biosynthesis.
    J Bacteriol. 2000 Dec;182(24):6958-63 PMID: 11092856
  6. Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis.
    Curr Opin Chem Biol. 2000 Dec;4(6):632-8 PMID: 11102867
  7. Identification of essential amino acids in the bacterial alpha -mannosyltransferase aceA.
    J Biol Chem. 2000 Dec 22;275(51):40568-75 PMID: 11001941
  8. Characterization of a putative alpha-mannosyltransferase involved in phosphatidylinositol trimannoside biosynthesis in Mycobacterium tuberculosis.
    Biochem J. 2002 May 1;363(Pt 3):437-47 PMID: 11964144
  9. ATP-dependent L-cysteine:1D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase, mycothiol biosynthesis enzyme MshC, is related to class I cysteinyl-tRNA synthetases.
    Biochemistry. 2002 Jun 4;41(22):6885-90 PMID: 12033919
  10. Sucrose metabolism: Anabaena sucrose-phosphate synthase and sucrose-phosphate phosphatase define minimal functional domains shuffled during evolution.
    FEBS Lett. 2002 Apr 24;517(1-3):19-23 PMID: 12062401
  11. Identification of the mycothiol synthase gene (mshD) encoding the acetyltransferase producing mycothiol in actinomycetes.
    Arch Microbiol. 2002 Nov;178(5):331-7 PMID: 12375100
  12. Mycothiol-deficient Mycobacterium smegmatis mutants are hypersensitive to alkylating agents, free radicals, and antibiotics.
    Antimicrob Agents Chemother. 2002 Nov;46(11):3348-55 PMID: 12384335
  13. Mycothiol biochemistry.
    Arch Microbiol. 2002 Dec;178(6):388-94 PMID: 12420157
  14. Association of mycothiol with protection of Mycobacterium tuberculosis from toxic oxidants and antibiotics.
    Mol Microbiol. 2003 Mar;47(6):1723-32 PMID: 12622824
  15. Cloning, characterization, and DNA sequence of the rfaLK region for lipopolysaccharide synthesis in Salmonella typhimurium LT2.
    J Bacteriol. 1991 Nov;173(22):7151-63 PMID: 1657881
  16. Thiols of intracellular pathogens. Identification of ovothiol A in Leishmania donovani and structural analysis of a novel thiol from Mycobacterium bovis.
    Eur J Biochem. 1994 Aug 15;224(1):203-13 PMID: 8076641
  17. Prospects for new interventions in the treatment and prevention of mycobacterial disease.
    Annu Rev Microbiol. 1995;49:641-73 PMID: 8561475
  18. Distribution of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes.
    J Bacteriol. 1996 Apr;178(7):1990-5 PMID: 8606174
  19. Towards a classification of glycosyltransferases based on amino acid sequence similarities: prokaryotic alpha-mannosyltransferases.
    Biochem J. 1996 Aug 15;318 ( Pt 1):133-8 PMID: 8761462
  20. A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities.
    Biochem J. 1997 Sep 15;326 ( Pt 3):929-39 PMID: 9334165
  21. The pimB gene of Mycobacterium tuberculosis encodes a mannosyltransferase involved in lipoarabinomannan biosynthesis.
    J Biol Chem. 1999 Oct 29;274(44):31625-31 PMID: 10531370
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.11.3476-3479.2003
Published
2003-06-00
Pages
3476-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC155378
Subset
IM
Grants
NIAID NIH HHS · R01 AI049174 · United States
NIAID NIH HHS · R21 AI049174 · United States
NIAID NIH HHS · AI49174 · United States
FIC NIH HHS · TW00976 · United States
External Links
PubMed source DOI: 10.1128/JB.185.11.3476-3479.2003 PMC Full Text View journal details
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