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PMID: 12756535 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The GATE retrotransposon in Drosophila melanogaster: mobility in heterochromatin and aspects of its expression in germline tissues.

Molecular genetics and genomics : MGG ·Vol. 269 ·No. 2 ·2003-05-00 ·Pages 234-42

Kogan GL, Tulin AV, Aravin AA, Abramov YA, Kalmykova AI, Maisonhaute C, Gvozdev VA

Abstract

A full-length copy of the retrotransposon GATE was identified as an insertion in the tandemly repeated, heterochromatic, Stellate genes, which are expressed in the testis of Drosophila melanogaster. Sequencing of this heterochromatic GATE copy revealed that it is closely related to the BEL retrotransposon, a representative of the recently defined BEL-like group of LTR retrotransposons. This copy contains identical LTRs, indicating that the insertion is a recent event. By contrast, the euchromatic part of the D. melanogaster genome contains only profoundly damaged GATE copies or fragments of the transposon. The preferential localization of GATE sequences in heterochromatin was confirmed for the other species in the melanogaster subgroup. The level of GATE expression is dramatically increased in ovaries, but not in testes, of spn-E(1) homozygous flies. We speculate that spn-E is involved in the silencing of GATE via an RNA interference mechanism.

MeSH Terms
Amino Acid Sequence Animals DNA/metabolism DNA Transposable Elements Drosophila melanogaster/genetics Female Heterochromatin/metabolism In Situ Hybridization Male Molecular Sequence Data Mutation Ovary/metabolism Phylogeny Protein Structure, Tertiary Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Species Specificity Testis/metabolism
Chemicals
DNA Transposable Elements Heterochromatin DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kogan G L
Department of Animal Molecular Genetics, Institute of Molecular Genetics, 123182 Moscow, Russia.
Tulin A V
Aravin A A
Abramov Yu A
Kalmykova A I
Maisonhaute C
Gvozdev V A
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Article Info
Journal
Molecular genetics and genomics : MGG
Abbr.
Mol Genet Genomics
ISSN
1617-4615
DOI
10.1007/s00438-003-0827-1
Published
2003-05-00
Epub
2003-00-14
Pages
234-42
Language
English
Region
Germany
NLM ID
101093320
Subset
IM
External Links
PubMed source DOI: 10.1007/s00438-003-0827-1 View journal details
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