A membrane permeability test for the detection of cell surface antigens.

Immunology ·Vol. 29 ·No. 5 ·1975-11-00 ·Pages 803-11

Kurth R, Medley G

Abstract

A convenient microtest is described which utilizes antibody-mediated release of [14C]nicotinamide ([14C]NA) from target cells for the detection of cell surface antigens. This test is considerably more sensitive and faster than the widely used 51Cr release test because most of the [14C]NA is rapidly released from the target cells in the initial phase of membrane permeability changes induced by activated complement, as distinct from the colloid osmotic phase of complement-mediated cytolysis.

MeSH Terms

Animals Antigen-Antibody Reactions Antigens Antigens, Neoplasm Cell Line Cell Membrane/immunology Cell Membrane Permeability Complement System Proteins Cytotoxicity Tests, Immunologic Fibroblasts/immunology,metabolism Methods Mice Niacinamide/immunology,metabolism Sarcoma, Experimental/immunology

Chemicals

Antigens Antigens, Neoplasm Niacinamide Complement System Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kurth R

Medley G

References (12)

12 references, click to expand

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Article Info

Journal

Immunology

Abbr.

Immunology

ISSN

0019-2805

Published

1975-11-00

Pages

803-11

Language

English

Region

England

NLM ID

0374672

PMCID

PMC1445986

Subset

External Links

PubMed source PMC Full Text View journal details

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A membrane permeability test for the detection of cell surface antigens.

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