The glycoprotease of Pasteurella haemolytica A1 eliminates binding of myeloid cells to P-selectin but not to E-selectin.

Biochemical and biophysical research communications ·Vol. 188 ·No. 2 ·1992-10-30 ·Pages 760-6

Steininger CN, Eddy CA, Leimgruber RM, Mellors A, Welply JK

Abstract

HL-60 cells and neutrophils treated with the glycoprotease from Pasteurella haemolytica A1, an enzyme which is specific for O-sialoglycoproteins, were found to be incapable of binding P-selectin but still bound E-selectin. Comparative analysis of [35-S] cysteine labeled proteins from HL-60 cells by 2-dimensional electrophoresis indicated that two major proteins with M(r) 100 and 115 kd were significantly removed from cells which had been treated.

MeSH Terms

Antigens, CD/metabolism Cell Adhesion Molecules/metabolism Cysteine/metabolism E-Selectin Electrophoresis, Gel, Two-Dimensional Humans Leukemia, Promyelocytic, Acute Leukocyte Common Antigens/metabolism Leukosialin Mannheimia haemolytica/enzymology Membrane Glycoproteins/biosynthesis,isolation & purification,metabolism Metalloendopeptidases/metabolism,pharmacology Neutrophils/metabolism P-Selectin Platelet Membrane Glycoproteins/metabolism Protein Binding Receptors, Lymphocyte Homing/metabolism Sialoglycoproteins/biosynthesis,isolation & purification,metabolism Tumor Cells, Cultured

Chemicals

Antigens, CD Cell Adhesion Molecules E-Selectin Leukosialin Membrane Glycoproteins P-Selectin Platelet Membrane Glycoproteins Receptors, Lymphocyte Homing SPN protein, human Sialoglycoproteins Leukocyte Common Antigens Metalloendopeptidases O-sialoglycoprotein endopeptidase Cysteine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Steininger C N

Monsanto Corporate Research, Monsanto Company, St. Louis, MO 63167.

Eddy C A

Leimgruber R M

Mellors A

Welply J K

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1016/0006-291x(92)91121-6

Published

1992-10-30

Pages

760-6

Language

English

Region

United States

NLM ID

0372516

Subset

External Links

PubMed source DOI: 10.1016/0006-291x(92)91121-6 View journal details

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The glycoprotease of Pasteurella haemolytica A1 eliminates binding of myeloid cells to P-selectin but not to E-selectin.

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