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PMID: 12801956 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Live probiotics protect intestinal epithelial cells from the effects of infection with enteroinvasive Escherichia coli (EIEC).

Gut ·Vol. 52 ·No. 7 ·2003-07-00 ·Pages 988-97

Resta-Lenert S, Barrett KE

Abstract

The colonic epithelium maintains a life long reciprocally beneficial interaction with the colonic microbiota. Disruption is associated with mucosal injury. We hypothesised that probiotics may limit epithelial damage induced by enteroinvasive pathogens, and promote restitution. Human intestinal epithelial cell lines (HT29/cl.19A and Caco-2) were exposed to enteroinvasive Escherichia coli (EIEC 029:NM), and/or probiotics (Streptococcus thermophilus (ST), ATCC19258, and Lactobacillus acidophilus (LA), ATCC4356). Infected cells and controls were assessed for transepithelial resistance, chloride secretory responses, alterations in cytoskeletal and tight junctional proteins, and responses to epidermal growth factor (EGF) stimulation. Exposure of cell monolayers to live ST/LA, but not to heat inactivated ST/LA, significantly limited adhesion, invasion, and physiological dysfunction induced by EIEC. Antibiotic killed ST/LA reduced adhesion somewhat but were less effective in limiting the consequences of EIEC invasion of cell monolayers. Furthermore, live ST/LA alone increased transepithelial resistance, contrasting markedly with the fall in resistance evoked by EIEC infection, which could also be blocked by live ST/LA. The effect of ST/LA on resistance was accompanied by maintenance (actin, ZO-1) or enhancement (actinin, occludin) of cytoskeletal and tight junctional protein phosphorylation. ST/LA had no effect on chloride secretion by themselves but reversed the increase in basal secretion evoked by EIEC. EIEC also reduced the ability of EGF to activate its receptor, which was reversed by ST/LA. Live ST/LA interact with intestinal epithelial cells to protect them from the deleterious effect of EIEC via mechanisms that include, but are not limited to, interference with pathogen adhesion and invasion. Probiotics likely also enhance the barrier function of naïve epithelial cells not exposed to any pathogen.

MeSH Terms
Cell Adhesion/physiology Cell Line Cell Membrane Permeability/physiology Chlorides/metabolism Colonic Diseases/metabolism,microbiology,prevention & control Colony Count, Microbial Cytoskeleton/metabolism Diarrhea/metabolism,microbiology,prevention & control Epithelial Cells/metabolism,microbiology ErbB Receptors/metabolism Escherichia coli Infections/metabolism,microbiology,prevention & control Humans Immunoblotting/methods Inflammatory Bowel Diseases/metabolism,microbiology,prevention & control Lactobacillus acidophilus Membrane Proteins/metabolism Phosphorylation Probiotics/therapeutic use Streptococcus
Chemicals
Chlorides Membrane Proteins ErbB Receptors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Resta-Lenert S
Department of Medicine, School of Medicine, University of California-San Diego, UCSD Medical Center 8414, 200 West Arbor Drive, San Diego, CA 92103, USA. [email protected]
Barrett K E
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Article Info
Journal
Gut
Abbr.
Gut
ISSN
0017-5749
DOI
10.1136/gut.52.7.988
Email
[email protected]
Published
2003-07-00
Pages
988-97
Language
English
Region
England
NLM ID
2985108R
PMCID
PMC1773702
Subset
IM
Grants
NIDDK NIH HHS · P01 DK035108 · United States
NIDDK NIH HHS · DK 35108 · United States
External Links
PubMed source DOI: 10.1136/gut.52.7.988 PMC Full Text View journal details
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