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PMID: 12805204 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Three-dimensional structure of the M-MuLV CA protein on a lipid monolayer: a general model for retroviral capsid assembly.

The EMBO journal ·Vol. 22 ·No. 12 ·2003-06-16 ·Pages 2886-92

Ganser BK, Cheng A, Sundquist WI, Yeager M

Abstract

Although retroviruses from different genera form morphologically distinct capsids, we have proposed that all of these structures are composed of similar hexameric arrays of capsid (CA) protein subunits and that their distinct morphologies reflect different distributions of pentameric declinations that allow the structures to close. Consistent with this model, CA proteins from both HIV-1 and Rous sarcoma virus (RSV) form similar hexagonal lattices. However, recent structural studies have suggested that the Moloney murine leukemia virus (M-MuLV) CA protein may assemble differently. We now report an independent three-dimensional reconstruction of two-dimensional crystals of M-MuLV CA. This new reconstruction reveals a hexameric lattice that is similar to those formed by HIV-1 and RSV CA, supporting a generalized model for retroviral capsid assembly.

MeSH Terms
Animals Capsid Proteins/chemistry,genetics,metabolism,ultrastructure Crystallography, X-Ray Lipid Metabolism Lipids/chemistry Mice Models, Molecular Moloney murine leukemia virus/metabolism,ultrastructure Protein Structure, Quaternary Virus Replication
Chemicals
Capsid Proteins Lipids
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ganser Barbie K
Department of Biochemistry, University of Utah, 20 North 1900 East, Salt Lake City, UT 84132, USA.
Cheng Anchi
Sundquist Wesley I
Yeager Mark
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg276
Published
2003-06-16
Pages
2886-92
Language
English
Region
England
NLM ID
8208664
PMCID
PMC162131
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg276 PMC Full Text View journal details
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