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PMID: 1280832 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Marked increase in nitric oxide synthase mRNA in rat dorsal root ganglia after peripheral axotomy: in situ hybridization and functional studies.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 23 ·1992-12-01 ·Pages 11617-21

Verge VM, Xu Z, Xu XJ, Wiesenfeld-Hallin Z, Hökfelt T

Abstract

Using in situ hybridization, we studied nitric oxide (NO) synthase (EC 1.14.23.-) mRNA in lumbar dorsal root ganglia after peripheral transection of the sciatic nerve in rats. The effect of the NO synthase inhibitor N omega-nitro-L-arginine methyl ester on the nociceptive flexor reflex was also studied in axotomized rats. Nerve section induced a dramatic increase in number of NO synthase mRNA-positive cells in the ipsilateral dorsal root ganglia. In some of these cells the peptides galanin and/or vasoactive intestinal polypeptide and/or neuropeptide Y were also strongly up-regulated. Intravenous administration of nitro-L-arginine methyl ester blocked spinal hyperexcitability at much lower dosages in axotomized than in normal animals. The results suggest involvement of NO in the function of lumbar sensory neurons, especially after axotomy, perhaps preferentially at peripheral sites.

MeSH Terms
Amino Acid Oxidoreductases/genetics Animals Ganglia, Spinal/enzymology Gene Expression In Situ Hybridization Male Nerve Regeneration Neuronal Plasticity Nitric Oxide/metabolism Nitric Oxide Synthase Peripheral Nerves/physiology RNA, Messenger/genetics Rats Rats, Sprague-Dawley Vasoactive Intestinal Peptide/genetics
Chemicals
RNA, Messenger Nitric Oxide Vasoactive Intestinal Peptide Nitric Oxide Synthase Amino Acid Oxidoreductases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Verge V M
Department of Histology and Neurobiology, Karolinska Institute, Stockholm, Sweden.
Xu Z
Xu X J
Wiesenfeld-Hallin Z
Hökfelt T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.23.11617
Published
1992-12-01
Pages
11617-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50604
Subset
IM
Grants
PHS HHS · 43230 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.23.11617 PMC Full Text View journal details
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