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PMID: 1281815 Published · ppublish English Journal Article

The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope.

The Journal of cell biology ·Vol. 119 ·No. 6 ·1992-12-00 ·Pages 1441-9

Wozniak RW, Blobel G

Abstract

The glycoprotein gp210 is located in the "pore membrane," a specialized domain of the nuclear envelope to which the nuclear pore complex (NPC) is anchored. gp210 contains a large cisternal domain, a single transmembrane segment (TM), and a COOH-terminal, 58-amino acid residue cytoplasmic tail (CT) (Wozniak, R. W., E. Bartnik, and G. Blobel. 1989. J. Cell Biol. 108:2083-2092; Greber, U. F., A. Senior, and L. Gerace. 1990. EMBO (Eur. Mol. Biol. Organ.) J. 9:1495-1502). To locate determinants for sorting of gp210 to the pore membrane, we constructed various cDNAs coding for wild-type, mutant, and chimeric gp210, and monitored localization of the expressed protein in 3T3 cells by immunofluorescence microscopy using appropriate antibodies. The large cisternal domain of gp210 (95% of its mass) did not reveal any sorting determinants. Surprisingly, the TM of gp210 is sufficient for sorting to the pore membrane. The CT also contains a sorting determinant, but it is weaker than that of the TM. We propose specific lateral association of the transmembrane helices of two proteins to yield either a gp210 homodimer or a heterodimer of gp210 and another protein. The cytoplasmically oriented tails of these dimers may bind cooperatively to the adjacent NPCs. In addition, we demonstrate that gp210 co-localizes with cytoplasmically dispersed nucleoporins, suggesting a cytoplasmic association of these components.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Transport CD8 Antigens/genetics,metabolism DNA Mutational Analysis Epitopes Fibroblasts/metabolism Fluorescent Antibody Technique Membrane Glycoproteins/genetics,isolation & purification,metabolism Mice Mice, Inbred BALB C Molecular Sequence Data Nuclear Envelope/metabolism Nuclear Pore Complex Proteins Nuclear Proteins/genetics,isolation & purification,metabolism Protein Sorting Signals/genetics,isolation & purification,metabolism Recombinant Fusion Proteins/genetics,isolation & purification,metabolism Structure-Activity Relationship
Chemicals
CD8 Antigens Epitopes Membrane Glycoproteins Nuclear Pore Complex Proteins Nuclear Proteins Nup210 protein, mouse Protein Sorting Signals Recombinant Fusion Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wozniak R W
Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021.
Blobel G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.119.6.1441
Published
1992-12-00
Pages
1441-9
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289754
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.119.6.1441 PMC Full Text View journal details
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