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PMID: 12819094 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Candida albicans septin mutants are defective for invasive growth and virulence.

Infection and immunity ·Vol. 71 ·No. 7 ·2003-07-00 ·Pages 4045-51

Warenda AJ, Kauffman S, Sherrill TP, Becker JM, Konopka JB

Abstract

Hyphal growth of Candida albicans is implicated as an important virulence factor for this opportunistic human pathogen. Septin proteins, a family of cytoskeletal elements that regulate membrane events and are important for proper morphogenesis of C. albicans, were examined for their role in tissue invasion and virulence in the mouse model of systemic infection. In vitro, septin mutants are only mildly defective for hyphal growth in liquid culture but display pronounced defects for invasive growth into agar. In vivo, the septin mutants were found to exhibit attenuated virulence. However, mice infected with the mutants displayed high fungal burdens in their kidneys without obvious symptoms of disease. Histological examination of infected kidneys revealed defects in organ invasion for the cdc10 Delta and cdc11 Delta deletion mutants, which displayed both reduced tissue penetration and noninvasive fungal masses. Thus, the septin proteins are necessary for invasive growth, which appears to be more important to the successful pathogenesis of C. albicans than hyphal growth alone.

MeSH Terms
Agar Animals Candida albicans/growth & development,pathogenicity Cell Cycle Proteins/physiology Cytoskeletal Proteins/physiology GTP Phosphohydrolases Hyphae/growth & development Kidney/microbiology Macrophages/microbiology Male Membrane Proteins Mice Mice, Inbred ICR Mutation Saccharomyces cerevisiae Proteins/physiology Schizosaccharomyces pombe Proteins Septins Transcription Factors Virulence
Chemicals
CDC11 protein, S cerevisiae Cell Cycle Proteins Cytoskeletal Proteins Membrane Proteins SHS1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Transcription Factors cdc10 protein, S pombe Agar CDC10 protein, S cerevisiae GTP Phosphohydrolases Sept7 protein, mouse Septins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Warenda Amy J
Department of Molecular Genetics and Microbiology, State University of New York, Stony Brook, New York 11794-5222, USA.
Kauffman Sarah
Sherrill Taylor P
Becker Jeffrey M
Konopka James B
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.71.7.4045-4051.2003
Published
2003-07-00
Pages
4045-51
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC161988
Subset
IM
Grants
NIAID NIH HHS · R01 AI047837 · United States
NIAID NIH HHS · R01 AI47837 · United States
External Links
PubMed source DOI: 10.1128/IAI.71.7.4045-4051.2003 PMC Full Text View journal details
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