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PMID: 12821642 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Yeast osmosensor Sln1 and plant cytokinin receptor Cre1 respond to changes in turgor pressure.

The Journal of cell biology ·Vol. 161 ·No. 6 ·2003-06-23 ·Pages 1035-40

Reiser V, Raitt DC, Saito H

Abstract

Very little is known about how cellular osmosensors monitor changes in osmolarity of the environment. Here, we report that in yeast, Sln1 osmosensor histidine kinase monitors changes in turgor pressures. Reductions in turgor caused by either hyperosmotic stress, nystatin, or removal of cell wall activate MAPK Hog1 specifically through the SLN1 branch, but not through the SHO1 branch of the high osmolarity glycerol pathway. The integrity of the periplasmic region of Sln1 was essential for its sensor function. We found that activity of the plant histidine kinase cytokinin response 1 (Cre1) is also regulated by changes in turgor pressure, in a manner identical to that of Sln1, in the presence of cytokinin. We propose that Sln1 and Cre1 are turgor sensors, and that similar turgor-sensing mechanisms might regulate hyperosmotic stress responses both in yeast and plants.

MeSH Terms
Arabidopsis Proteins/metabolism Cell Wall/metabolism Cytokinins/metabolism,pharmacology Glycerol/metabolism Intracellular Signaling Peptides and Proteins Intracranial Pressure/physiology Mitogen-Activated Protein Kinases/metabolism Nystatin/pharmacology Osmotic Pressure Protein Kinases/metabolism Receptors, Cell Surface/metabolism Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins/metabolism Water-Electrolyte Balance/physiology
Chemicals
Arabidopsis Proteins Cytokinins Intracellular Signaling Peptides and Proteins Receptors, Cell Surface Saccharomyces cerevisiae Proteins Nystatin Protein Kinases HOG1 protein, S cerevisiae Mitogen-Activated Protein Kinases SLN1 protein, S cerevisiae WOL protein, Arabidopsis Glycerol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Reiser VladimIr
Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA.
Raitt Desmond C
Saito Haruo
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30 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200301099
Published
2003-06-23
Pages
1035-40
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2172993
Subset
IM
Grants
NIGMS NIH HHS · GM56699 · United States
External Links
PubMed source DOI: 10.1083/jcb.200301099 PMC Full Text View journal details
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