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PMID: 1282314 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Selection of a Pseudomonas cepacia strain constitutive for the degradation of trichloroethylene.

Applied and environmental microbiology ·Vol. 58 ·No. 12 ·1992-12-00 ·Pages 3977-83

Shields MS, Reagin MJ

Abstract

Tn5 insertion mutants of Pseudomonas cepacia G4 that were unable to degrade trichloroethylene (TCE), toluene, or phenol or to transform m-trifluoromethyl phenol (TFMP) to 7,7,7-trifluoro-2-hydroxy-6-oxo-2,4-heptadienoic acid (TFHA) were produced. Spontaneous reversion to growth on phenol or toluene as the sole source of carbon was observed in one mutant strain, G4 5223, at a frequency of approximately 1 x 10(-4) per generation. One such revertant, G4 5223-PR1, metabolized TFMP to TFHA and degraded TCE. Unlike wild-type G4, G4 5223-PR1 constitutively metabolized both TFMP and TCE without aromatic induction. G4 5223-PR1 also degraded cis-1,2-dichloroethylene, trans-1,2-dichloroethylene, and 1,1-dichloroethylene and oxidized naphthalene to alpha naphthol constitutively. G4 5223-PR1 exhibited a slight retardation in growth rate at TCE concentrations of > or = 530 microM, whereas G4 (which was unable to metabolize TCE under the same noninducing growth conditions) remained unaffected. The constitutive degradative phenotype of G4 5223-PR1 was completely stable through 100 generations of nonselective growth.

MeSH Terms
Biodegradation, Environmental Burkholderia cepacia/genetics,growth & development,metabolism DNA Transposable Elements Hydrocarbons, Chlorinated/metabolism Mutagenesis, Insertional Trichloroethylene/metabolism Water Pollutants, Chemical/metabolism
Chemicals
DNA Transposable Elements Hydrocarbons, Chlorinated Water Pollutants, Chemical Trichloroethylene
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shields M S
Center for Environmental Diagnostics and Bioremediation, University of West Florida, Pensacola 32514-5751.
Reagin M J
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.58.12.3977-3983.1992
Published
1992-12-00
Pages
3977-83
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC183214
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.58.12.3977-3983.1992 PMC Full Text View journal details
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