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PMID: 1282488 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities.

FEMS microbiology letters ·Vol. 100 ·No. 1-3 ·1992-12-15 ·Pages 59-65

Rochelle PA, Fry JC, Parkes RJ, Weightman AJ

Abstract

A protocol was devised which permitted the extraction of DNA from deep marine sediments up to 503 m below the sea floor. These sediments have been laid down over the last 3 million years. 16S rRNA gene sequences were amplified from the DNA by the polymerase chain reaction. The details of the successful extraction and polymerase chain reaction methodology varied between samples from different depths. This emphasizes the attention to detail required to allow the diversity of bacteria in these deep sediments to be studied.

MeSH Terms

Bacteria/genetics,isolation & purification Base Sequence DNA, Bacterial/genetics,isolation & purification Genetic Variation Marine Biology Molecular Sequence Data Polymerase Chain Reaction/methods RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Water Microbiology

Chemicals

DNA, Bacterial RNA, Bacterial RNA, Ribosomal, 16S

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Rochelle P A

School of Pure and Applied Biology, University of Wales College of Cardiff, UK.

Fry J C

Parkes R J

Weightman A J

Article Info

Journal

FEMS microbiology letters

Abbr.

FEMS Microbiol Lett

ISSN

0378-1097

DOI

10.1111/j.1574-6968.1992.tb14019.x

Published

1992-12-15

Pages

59-65

Language

English

Region

England

NLM ID

7705721

Subset

External Links

PubMed source DOI: 10.1111/j.1574-6968.1992.tb14019.x View journal details

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DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities.

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