Home LiteratureArticle Details
PMID: 12825685 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A maize QTL for silk maysin levels contains duplicated Myb-homologous genes which jointly regulate flavone biosynthesis.

Plant molecular biology ·Vol. 52 ·No. 1 ·2003-05-00 ·Pages 1-15

Zhang P, Wang Y, Zhang J, Maddock S, Snook M, Peterson T

Abstract

The maize p1 locus coincides with a major QTL (quantitative trait locus) determining levels of maysin, a C-glycosyl flavone that deters feeding by corn ear-worm. The p1 gene is tightly linked with a second gene, p2, and both genes encode similar Myb-domain proteins. We show here that maize cell cultures transformed with either the p1 or p2 genes expressed under a constitutive promoter accumulate transcripts for flavonoid biosynthetic genes, and synthesize phenylpropanoids and C-glycosyl flavones related to maysin. Additionally, maize plants that are deleted for the p1 gene have reduced maysin levels and moderate silk-browning reaction, whereas plants with a deletion of both p1 and p2 have non-detectable silk maysin and non-browning silks. We conclude that both p1 and p2 induce maysin biosynthesis in silk, although the two genes differ in their expression and pigmentation effects in other tissues. These results show that a QTL for flavone biosynthesis actually comprises two tightly linked genes with related functions.

MeSH Terms
Flavones Flavonoids/biosynthesis,metabolism Gene Deletion Gene Expression Regulation, Plant Genes, Duplicate/genetics Genes, Plant/genetics Glucosides/metabolism Mutation Plant Proteins/genetics Proto-Oncogene Proteins c-myb/genetics Quantitative Trait Loci/genetics Transformation, Genetic Zea mays/cytology,genetics,metabolism
Chemicals
Flavones Flavonoids Glucosides Plant Proteins Proto-Oncogene Proteins c-myb maysin flavone
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zhang Peifen
Department of Zoology and Genetics, Iowa State University, Ames, IA 50011, USA.
Wang Yibin
Zhang Jianbo
Maddock Sheila
Snook Maurice
Peterson Thomas
References (37)
37 references, click to expand
  1. Preservation of duplicate genes by complementary, degenerative mutations.
    Genetics. 1999 Apr;151(4):1531-45 PMID: 10101175
  2. Tissue-specific patterns of a maize Myb transcription factor are epigenetically regulated.
    Plant J. 2001 Sep;27(5):467-78 PMID: 11576430
  3. Alpha-tubulin gene family of maize (Zea mays L.). Evidence for two ancient alpha-tubulin genes in plants.
    J Mol Biol. 1992 Sep 5;227(1):81-96 PMID: 1522603
  4. Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants.
    Transgenic Res. 1996 May;5(3):213-8 PMID: 8673150
  5. Genome rearrangements by nonlinear transposons in maize.
    Genetics. 1999 Nov;153(3):1403-10 PMID: 10545468
  6. Restriction fragment length polymorphism markers associated with silk maysin, antibiosis to corn earworm (Lepidoptera: Noctuidae) larvae, in a dent and sweet corn cross.
    J Econ Entomol. 2001 Apr;94(2):564-71 PMID: 11332855
  7. Bronze-2 gene of maize: reconstruction of a wild-type allele and analysis of transcription and splicing.
    Plant Cell. 1990 Nov;2(11):1039-49 PMID: 1967051
  8. Identification of the residues in the Myb domain of maize C1 that specify the interaction with the bHLH cofactor R.
    Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13579-84 PMID: 11095727
  9. Maize anthocyanin regulatory gene pl is a duplicate of c1 that functions in the plant.
    Plant Cell. 1993 Dec;5(12):1795-805 PMID: 8305872
  10. Engineering secondary metabolism in maize cells by ectopic expression of transcription factors.
    Plant Cell. 1998 May;10(5):721-40 PMID: 9596632
  11. Ac induces homologous recombination at the maize P locus.
    Genetics. 1991 May;128(1):163-73 PMID: 1648001
  12. white anther: A petunia mutant that abolishes pollen flavonol accumulation, induces male sterility, and is complemented by a chalcone synthase transgene
    Plant Physiol. 1999 Jun;120(2):615-22 PMID: 10364414
  13. Flavonols are not essential for fertilization in Arabidopsis thaliana.
    Plant Mol Biol. 1996 Dec;32(6):1155-8 PMID: 9002614
  14. A segmental gene duplication generated differentially expressed myb-homologous genes in maize.
    Plant Cell. 2000 Dec;12(12):2311-2322 PMID: 11148280
  15. Alternatively spliced products of the maize P gene encode proteins with homology to the DNA-binding domain of myb-like transcription factors.
    Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):4587-91 PMID: 2052542
  16. Plant polyphenols. 4. Hydroxycinnamic acid-sugar derivatives.
    Biochem J. 1961 Nov;81:242-50 PMID: 13904579
  17. Genetic and developmental control of anthocyanin biosynthesis.
    Annu Rev Genet. 1991;25:173-99 PMID: 1839877
  18. Identification of a naturally occurring inhibitor of the conversion of 1-aminocyclopropane-1-carboxylic Acid to ethylene by carnation microsomes.
    Plant Physiol. 1989 Apr;89(4):1053-9 PMID: 16666663
  19. Complex structure of a maize Myb gene promoter: functional analysis in transgenic plants.
    Plant J. 2000 Jun;22(6):471-82 PMID: 10886767
  20. Genetic mechanisms underlying apimaysin and maysin synthesis and corn earworm antibiosis in maize (Zea mays L.).
    Genetics. 1998 Aug;149(4):1997-2006 PMID: 9691053
  21. A maize gene controlling silk browning in response to wounding.
    Genetics. 1971 Dec;69(4):491-8 PMID: 17248552
  22. The probability of duplicate gene preservation by subfunctionalization.
    Genetics. 2000 Jan;154(1):459-73 PMID: 10629003
  23. Antisense inhibition of flavonoid biosynthesis in petunia anthers results in male sterility.
    Plant Cell. 1992 Mar;4(3):253-62 PMID: 1498595
  24. Sequence of three bronze alleles of maize and correlation with the genetic fine structure.
    Genetics. 1988 May;119(1):185-97 PMID: 3396861
  25. Influence of transposable elements on the structure and function of the A1 gene of Zea mays.
    EMBO J. 1987 Feb;6(2):287-94 PMID: 15981326
  26. Quantitative trait loci and metabolic pathways: genetic control of the concentration of maysin, a corn earworm resistance factor, in maize silks.
    Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8820-5 PMID: 11607699
  27. A maize Myb homolog is encoded by a multicopy gene complex.
    Mol Gen Genet. 1998 Nov;260(4):372-80 PMID: 9870702
  28. Flavanone 3-hydroxylase transcripts and flavonol accumulation are temporally coordinate in maize anthers.
    Plant J. 1995 May;7(5):703-13 PMID: 7773305
  29. Hierarchical patterns of transgene expression indicate involvement of developmental mechanisms in the regulation of the maize P1-rr promoter.
    Genetics. 2000 Oct;156(2):839-46 PMID: 11014829
  30. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
    Nature. 2000 Dec 14;408(6814):796-815 PMID: 11130711
  31. Apparent redundancy in myb gene function provides gearing for the control of flavonoid biosynthesis in antirrhinum flowers.
    Plant Cell. 1996 Sep;8(9):1519-32 PMID: 8837506
  32. Ac insertion site affects the frequency of transposon-induced homologous recombination at the maize p1 locus.
    Genetics. 2000 Dec;156(4):2007-17 PMID: 11102391
  33. Biochemical complementation of chalcone synthase mutants defines a role for flavonols in functional pollen.
    Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7213-7 PMID: 11607312
  34. Probing the component structure of a maize gene with transposable elements.
    Science. 1980 Jun 27;208(4451):1457-9 PMID: 17796696
  35. Alleles of the maize P gene with distinct tissue specificities encode Myb-homologous proteins with C-terminal replacements.
    Plant Cell. 1996 Jul;8(7):1149-58 PMID: 8768374
  36. The myb-homologous P gene controls phlobaphene pigmentation in maize floral organs by directly activating a flavonoid biosynthetic gene subset.
    Cell. 1994 Feb 11;76(3):543-53 PMID: 8313474
  37. Expression profiling of the maize flavonoid pathway genes controlled by estradiol-inducible transcription factors CRC and P.
    Plant Cell. 2000 Jan;12(1):65-80 PMID: 10634908
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1023942819106
Published
2003-05-00
Pages
1-15
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1023942819106 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2