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PMID: 12867474 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Biochemical function of msl5 (pks8 plus pks17) in Mycobacterium tuberculosis H37Rv: biosynthesis of monomethyl branched unsaturated fatty acids.

Journal of bacteriology ·Vol. 185 ·No. 15 ·2003-08-00 ·Pages 4620-5

Dubey VS, Sirakova TD, Cynamon MH, Kolattukudy PE

Abstract

We show that the disruption of one of the mycocerosic acid synthase (mas)-like genes, msl5 (pks8 plus pks17) in Mycobacterium tuberculosis H37Rv generates a mutant incapable of producing monomethyl branched unsaturated C(16) to C(20) fatty acids that are minor constituents of acyltrehaloses and sulfolipids. The msl5 mutation did not cause any significant change in the acyl lipid composition and also did not affect growth in culture, in mouse alveolar macrophage cell line MH-S, or in the murine lung.

MeSH Terms
Acyl Coenzyme A/metabolism Acyltransferases/genetics,metabolism Animals Bacterial Proteins/genetics,metabolism Cell Line Fatty Acids, Unsaturated/biosynthesis,chemistry Lung/microbiology Macrophages, Alveolar/microbiology Mice Mice, Inbred C57BL Mutation Mycobacterium tuberculosis/genetics,metabolism,pathogenicity Tuberculosis, Pulmonary/microbiology Virulence
Chemicals
Acyl Coenzyme A Bacterial Proteins Fatty Acids, Unsaturated methylmalonyl-coenzyme A Acyltransferases mycocerosic acid synthase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dubey Vinod S
Biomolecular Science Center, University of Central Florida, Orlando, Florida 32816, USA.
Sirakova Tatiana D
Cynamon Michael H
Kolattukudy Pappachan E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.15.4620-4625.2003
Published
2003-08-00
Pages
4620-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC165776
Subset
IM
Grants
NIAID NIH HHS · R01 AI035272 · United States
NIAID NIH HHS · R01 AI046582 · United States
NIAID NIH HHS · AI35272-01 · United States
NIAID NIH HHS · AI46582-03 · United States
External Links
PubMed source DOI: 10.1128/JB.185.15.4620-4625.2003 PMC Full Text View journal details
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