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PMID: 12885640 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of calcium channel activity by lipid domain formation in planar lipid bilayers.

Biophysical journal ·Vol. 85 ·No. 2 ·2003-08-00 ·Pages 933-42

Cannon B, Hermansson M, Györke S, Somerharju P, Virtanen JA, Cheng KH

Abstract

The sarcoplasmic reticulum channel (ryanodine receptor) from cardiac myocytes was reconstituted into planar lipid bilayers consisting of 1-palmitoyl-2-oleoyl-phosphatidylethanolamine (POPE) and 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) in varying ratios. The channel activity parameters, i.e., open probability and average open time and its resolved short and long components, were determined as a function of POPE mole fraction (X(PE)) at 22.4 degrees C. Interestingly, all of these parameters exhibited a narrow and pronounced peak at X(PE) approximately 0.80. Differential scanning calorimetric measurements on POPE/POPC liposomes with increasing X(PE) indicated that the lipid bilayer enters a composition-driven transition from the liquid-crystalline state to the gel state at 22.4 degrees C when X(PE) approaches 0.80. Thus, the peaking of the reconstituted channel activity at X(PE) approximately 0.80 in the planar bilayer could result from the appearance of gel/liquid-crystalline domain boundaries at this POPE content. Lipid packing at domain boundaries is known to be looser as compared to the homogenous gel or liquid-crystalline state. We propose that the attractive potential of packing defects at lipid domain boundaries and entropic excluded-volume effects could result in the direct interactions of the transmembrane region of the channel protein with the lipid-packing defects at the lipid/protein interface, which could thus provide a favorable environment for the open state of the protein. The present findings indicate that the activity of the sarcoplasmic reticulum calcium channel could be modulated by lipid domain formation upon slight changes in membrane lipid composition in vivo.

MeSH Terms
Calcium Channels/chemistry Electric Capacitance Ion Channel Gating Lipid Bilayers/chemistry Membrane Fluidity Membrane Microdomains/chemistry Membrane Potentials Membranes, Artificial Molecular Conformation Phosphatidylcholines/chemistry Phosphatidylethanolamines/chemistry Protein Conformation Ryanodine Receptor Calcium Release Channel/chemistry Structure-Activity Relationship
Chemicals
Calcium Channels Lipid Bilayers Membranes, Artificial Phosphatidylcholines Phosphatidylethanolamines Ryanodine Receptor Calcium Release Channel 1-palmitoyl-2-oleoylphosphatidylethanolamine 1-palmitoyl-2-oleoylphosphatidylcholine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cannon Brian
Department of Physics, Texas Tech University, Lubbock, Texas 79409, USA.
Hermansson Martin
Györke Sandor
Somerharju Pentti
Virtanen Jorma A
Cheng Kwan Hon
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(03)74532-9
Published
2003-08-00
Pages
933-42
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1303214
Subset
IM
Grants
NHLBI NIH HHS · HL-52620 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(03)74532-9 PMC Full Text View journal details
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