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PMID: 12885912 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference.

Journal of virology ·Vol. 77 ·No. 16 ·2003-08-00 ·Pages 8957-61

Wiznerowicz M, Trono D

Abstract

RNA interference has emerged as a powerful technique to downregulate the expression of specific genes in cells and in animals, thus opening new perspectives in fields ranging from developmental genetics to molecular therapeutics. Here, we describe a method that significantly expands the potential of RNA interference by permitting the conditional suppression of genes in mammalian cells. Within a lentivirus vector background, we subjected the polymerase III promoter-dependent production of small interfering RNAs to doxycycline-controllable transcriptional repression. The resulting system can achieve the highly efficient and completely drug-inducible knockdown of cellular genes. As lentivirus vectors can stably transduce a wide variety of targets both in vitro and in vivo and can be used to generate transgenic animals, the present system should have broad applications.

MeSH Terms
Cell Line Doxycycline/pharmacology Flow Cytometry Genetic Vectors Humans Lentivirus/genetics RNA Interference RNA Polymerase III/metabolism
Chemicals
RNA Polymerase III Doxycycline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wiznerowicz Maciej
Department of Genetics and Microbiology, CMU, Faculty of Medicine, University of Geneva, 1211 Geneva 4, Switzerland.
Trono Didier
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
2003-08-00
Pages
8957-61
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC167245
Subset
IM
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