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PMID: 12892561 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Direct interaction of beta-dystroglycan with F-actin.

The Biochemical journal ·Vol. 375 ·No. Pt 2 ·2003-10-15 ·Pages 329-37

Chen YJ, Spence HJ, Cameron JM, Jess T, Ilsley JL, Winder SJ

Abstract

Dystroglycans are essential transmembrane adhesion receptors for laminin. Alpha-dystroglycan is a highly glycosylated extracellular protein that interacts with laminin in the extracellular matrix and the transmembrane region of beta-dystroglycan. Beta-dystroglycan, via its cytoplasmic tail, interacts with dystrophin and utrophin and also with the actin cytoskeleton. As a part of the dystrophin-glycoprotein complex of muscles, dystroglycan is also important in maintaining sarcolemmal integrity. Mutations in dystrophin that lead to Duchenne muscular dystrophy also lead to a loss of dystroglycan from the sarcolemma, and chimaeric mice lacking muscle dystroglycan exhibit a severe muscular dystrophy phenotype. Using yeast two-hybrid analysis and biochemical and cell biological studies, we show, in the present study, that the cytoplasmic tail of beta-dystroglycan interacts directly with F-actin and, furthermore, that it bundles actin filaments and induces an aberrant actin phenotype when overexpressed in cells.

MeSH Terms
Actins/genetics,metabolism,ultrastructure Amino Acid Sequence Animals Cell Line Cell Membrane/metabolism Cytoskeletal Proteins/genetics,metabolism Cytoskeleton/metabolism,ultrastructure Dystroglycans Electrophoresis, Polyacrylamide Gel Fibroblasts/cytology,metabolism Green Fluorescent Proteins Humans Luminescent Proteins/genetics,metabolism Membrane Glycoproteins/genetics,metabolism Membrane Proteins/metabolism Mice Microfilament Proteins/genetics,metabolism Microscopy, Electron Molecular Sequence Data Protein Binding Rabbits Rats Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics Sequence Homology, Amino Acid Two-Hybrid System Techniques Utrophin
Chemicals
Actins Cytoskeletal Proteins DAG1 protein, human Luminescent Proteins Membrane Glycoproteins Membrane Proteins Microfilament Proteins Recombinant Fusion Proteins Utrn protein, mouse Utrophin Dystroglycans Green Fluorescent Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chen Yun-Ju
Institute of Biomedical and Life Sciences, Davidson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK.
Spence Heather J
Cameron Jacqueline M
Jess Thomas
Ilsley Jane L
Winder Steven J
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
1470-8728
DOI
10.1042/BJ20030808
Published
2003-10-15
Pages
329-37
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1223702
Subset
IM
External Links
PubMed source DOI: 10.1042/BJ20030808 PMC Full Text View journal details
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