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PMID: 12897245 Published · ppublish English Journal Article

Activation tagging in tomato identifies a transcriptional regulator of anthocyanin biosynthesis, modification, and transport.

The Plant cell ·Vol. 15 ·No. 8 ·2003-08-00 ·Pages 1689-703

Mathews H, Clendennen SK, Caldwell CG, Liu XL, Connors K, Matheis N, Schuster DK, Menasco DJ, Wagoner W, Lightner J, Wagner DR

Abstract

We have developed a high-throughput T-DNA insertional mutagenesis program in tomato using activation tagging to identify genes that regulate metabolic pathways. One of the activation-tagged insertion lines (ant1) showed intense purple pigmentation from the very early stage of shoot formation in culture, reflecting activation of the biosynthetic pathway leading to anthocyanin accumulation. The purple coloration resulted from the overexpression of a gene that encodes a MYB transcription factor. Vegetative tissues of ant1 plants displayed intense purple color, and the fruit showed purple spotting on the epidermis and pericarp. The gene-to-trait relationship of ant1 was confirmed by the overexpression of ANT1 in transgenic tomato and in tobacco under the control of a constitutive promoter. Suppression subtractive hybridization and RNA hybridization analysis of the purple tomato plants indicated that the overexpression of ANT1 caused the upregulation of genes that encode proteins in both the early and later steps of anthocyanidin biosynthesis as well as genes involved in the glycosylation and transport of anthocyanins into the vacuole.

MeSH Terms
Amino Acid Sequence Anthocyanins/biosynthesis,chemistry,metabolism Base Sequence DNA, Bacterial/genetics DNA, Plant/genetics Genes, Plant Lycopersicon esculentum/anatomy & histology,genetics,metabolism Molecular Sequence Data Mutagenesis, Insertional Phenotype Pigmentation/genetics,physiology Plant Proteins/genetics Plants, Genetically Modified Sequence Homology, Amino Acid Transcriptional Activation
Chemicals
Anthocyanins DNA, Bacterial DNA, Plant Plant Proteins T-DNA
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Mathews Helena
Exelixis Plant Sciences, Inc., Portland, OR 97224-7744, USA. [email protected]
Clendennen Stephanie K
Caldwell Colby G
Liu Xing Liang
Connors Karin
Matheis Nikolaus
Schuster Debra K
Menasco D J
Wagoner Wendy
Lightner Jonathan
Wagner D Ry
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.012963
Email
[email protected]
Published
2003-08-00
Pages
1689-703
Language
English
Region
England
NLM ID
9208688
PMCID
PMC167162
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.012963 PMC Full Text View journal details
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