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PMID: 12907709 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts.

Nucleic acids research ·Vol. 31 ·No. 16 ·2003-08-15 ·Pages 4696-701

Mitsuzawa H, Kanda E, Ishihama A

Abstract

Rpb4-Rpb7, a dissociable subcomplex of RNA polymerase II (pol II), is required for transcription initiation. To understand the role of Rpb7 in transcription initiation or other processes in transcription, we carried out a two-hybrid screen for proteins that interact with Rpb7 of the fission yeast Schizosaccharomyces pombe. The screen identified the S.pombe homolog of the Saccharomyces cerevisiae Nrd1, an RNA-binding protein implicated in 3' end formation of small nucleolar and small nuclear RNAs transcribed by pol II. The S.pombe protein, named Seb1 for seven binding, was essential for cell viability, and bound directly to Rpb7 in vitro. Saccharomyces cerevisiae Rpb7 also interacted with Nrd1, indicating that the interaction is conserved in evolution. Glu166 and/or Asp167 of S.pombe Rpb7, residues near the C-terminus of the 172 amino acid protein, were found to be important for its interaction with Seb1. Our results suggest that Rpb7 may function to anchor a processing factor to the pol II apparatus, thereby coupling RNA processing to transcription. The role for Rpb7 is consistent with its location in the pol II complex determined by recent structural studies.

MeSH Terms
Amino Acid Sequence Binding Sites/genetics Cell Division/genetics Fungal Proteins/chemistry,genetics,metabolism Gene Expression Regulation, Fungal Membrane Proteins/genetics,metabolism Molecular Sequence Data Mutation Protein Binding Protein Structure, Tertiary/genetics Protein Subunits/chemistry,genetics,metabolism RNA Polymerase II/chemistry,genetics,metabolism RNA-Binding Proteins/genetics,metabolism SEC Translocation Channels Saccharomyces cerevisiae Proteins/genetics,metabolism Schizosaccharomyces/enzymology,genetics,metabolism Sequence Homology, Amino Acid Transcription, Genetic/genetics Two-Hybrid System Techniques Vesicular Transport Proteins
Chemicals
Fungal Proteins Membrane Proteins Protein Subunits RNA-Binding Proteins SEC Translocation Channels Saccharomyces cerevisiae Proteins Sbh1 protein, S cerevisiae Vesicular Transport Proteins RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mitsuzawa Hiroshi
Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan. [email protected]
Kanda Emi
Ishihama Akira
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkg688
Email
[email protected]
Published
2003-08-15
Pages
4696-701
Language
English
Region
England
NLM ID
0411011
PMCID
PMC169969
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkg688 PMC Full Text View journal details
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