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PMID: 12912901 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Specific protein targeting during cell differentiation: polarized localization of Fus1p during mating depends on Chs5p in Saccharomyces cerevisiae.

Eukaryotic cell ·Vol. 2 ·No. 4 ·2003-08-00 ·Pages 821-5

Santos B, Snyder M

Abstract

In budding yeast, chs5 mutants are defective in chitin synthesis and cell fusion during mating. Chs5p is a late-Golgi protein required for the polarized transport of the chitin synthase Chs3p to the membrane. Here we show that Chs5p is also essential for the polarized targeting of Fus1p, but not of other cell fusion proteins, to the membrane during mating.

MeSH Terms
Cell Compartmentation/genetics Cell Differentiation/genetics Cell Membrane/genetics,metabolism Cell Polarity/genetics Cells, Cultured Chitin/biosynthesis Chitin Synthase/genetics,metabolism Fungal Proteins/genetics,metabolism Membrane Fusion/genetics Membrane Proteins Mutation/genetics Protein Transport/genetics Recombinant Fusion Proteins/genetics,metabolism Reproduction/genetics Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins
Chemicals
FUS1 protein, S cerevisiae Fungal Proteins Membrane Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Chitin CHS5 protein, S cerevisiae Chitin Synthase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Santos Beatriz
Molecular, Cellular, and Developmental Biology Department, Yale University, New Haven, Connecticut 06520-8103, USA.
Snyder Michael
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19 references, click to expand
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Article Info
Journal
Eukaryotic cell
Abbr.
Eukaryot Cell
ISSN
1535-9778
DOI
10.1128/EC.2.4.821-825.2003
Published
2003-08-00
Pages
821-5
Language
English
Region
United States
NLM ID
101130731
PMCID
PMC178347
Subset
IM
Grants
NIGMS NIH HHS · R01 GM036494 · United States
NIGMS NIH HHS · GM36494 · United States
External Links
PubMed source DOI: 10.1128/EC.2.4.821-825.2003 PMC Full Text View journal details
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