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PMID: 12923101 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Unique degradation signal for ClpCP in Bacillus subtilis.

Journal of bacteriology ·Vol. 185 ·No. 17 ·2003-09-00 ·Pages 5275-8

Pan Q, Losick R

Abstract

Regulation of the cell-specific transcription factor sigma(F) in the spore-forming bacterium Bacillus subtilis involves the antisigma factor SpoIIAB. Contributing to the activation of sigma(F) is the degradation of SpoIIAB in a manner that depends on the protease ClpCP. Here we show that the three residues (LCN) located at the extreme C terminus of SpoIIAB are both necessary and sufficient for this degradation. We also report that the use of the LCN extension as a degradation signal for ClpCP is unique to SpoIIAB.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Bacillus subtilis/genetics,metabolism,physiology Bacterial Proteins/chemistry,genetics,metabolism Endopeptidase Clp Gene Expression Regulation, Bacterial Heat-Shock Proteins/genetics,metabolism Serine Endopeptidases/genetics,metabolism Signal Transduction Spores, Bacterial/physiology
Chemicals
Bacterial Proteins ClpC protein, Bacteria Heat-Shock Proteins SpoIIB protein, Bacillus subtilis spore-specific proteins, Bacillus Serine Endopeptidases Endopeptidase Clp Adenosine Triphosphatases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pan Qi
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
Losick Richard
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.17.5275-5278.2003
Published
2003-09-00
Pages
5275-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC181016
Subset
IM
Grants
NIGMS NIH HHS · R01 GM018568 · United States
NIGMS NIH HHS · R37 GM018568 · United States
NIGMS NIH HHS · GM 18568 · United States
External Links
PubMed source DOI: 10.1128/JB.185.17.5275-5278.2003 PMC Full Text View journal details
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