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PMID: 12925754 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

TINA interacts with the NIMA kinase in Aspergillus nidulans and negatively regulates astral microtubules during metaphase arrest.

Molecular biology of the cell ·Vol. 14 ·No. 8 ·2003-08-00 ·Pages 3169-79

Osmani AH, Davies J, Oakley CE, Oakley BR, Osmani SA

Abstract

The tinA gene of Aspergillus nidulans encodes a protein that interacts with the NIMA mitotic protein kinase in a cell cycle-specific manner. Highly similar proteins are encoded in Neurospora crassa and Aspergillus fumigatus. TINA and NIMA preferentially interact in interphase and larger forms of TINA are generated during mitosis. Localization studies indicate that TINA is specifically localized to the spindle pole bodies only during mitosis in a microtubule-dependent manner. Deletion of tinA alone is not lethal but displays synthetic lethality in combination with the anaphase-promoting complex/cyclosome mutation bimE7. At the bimE7 metaphase arrest point, lack of TINA enhanced the nucleation of bundles of cytoplasmic microtubules from the spindle pole bodies. These microtubules interacted to form spindles joined in series via astral microtubules as revealed by live cell imaging. Because TINA is modified and localizes to the spindle pole bodies at mitosis, and lack of TINA causes enhanced production of cytoplasmic microtubules at metaphase arrest, we suggest TINA is involved in negative regulation of the astral microtubule organizing capacity of the spindle pole bodies during metaphase.

MeSH Terms
Amino Acid Sequence Aspergillus nidulans/enzymology,genetics,metabolism Cell Cycle Cell Cycle Proteins Cloning, Molecular Fungal Proteins/genetics,metabolism Interphase Metaphase/genetics Microtubules/metabolism Molecular Sequence Data NIMA-Related Kinase 1 Protein Serine-Threonine Kinases/metabolism Spindle Apparatus/metabolism Two-Hybrid System Techniques
Chemicals
Cell Cycle Proteins Fungal Proteins NIMA-Related Kinase 1 Protein Serine-Threonine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Osmani Aysha H
Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, USA. [email protected]
Davies Jonathan
Oakley C Elizabeth
Oakley Berl R
Osmani Stephen A
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e02-11-0715
Email
[email protected]
Published
2003-08-00
Epub
2003-00-17
Pages
3169-79
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC181558
Subset
IM
Grants
NIGMS NIH HHS · R01 GM031837 · United States
NIGMS NIH HHS · R01 GM042564 · United States
NIGMS NIH HHS · GM 31837 · United States
NIGMS NIH HHS · GM 42564 · United States
External Links
PubMed source DOI: 10.1091/mbc.e02-11-0715 PMC Full Text View journal details
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