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PMID: 12939276 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Crystal structures of human DJ-1 and Escherichia coli Hsp31, which share an evolutionarily conserved domain.

The Journal of biological chemistry ·Vol. 278 ·No. 45 ·2003-11-07 ·Pages 44552-9

Lee SJ, Kim SJ, Kim IK, Ko J, Jeong CS, Kim GH, Park C, Kang SO, Suh PG, Lee HS, Cha SS

Abstract

Human DJ-1 and Escherichia coli Hsp31 belong to ThiJ/PfpI family, whose members contain a conserved domain. DJ-1 is associated with autosomal recessive early onset parkinsonism and Hsp31 is a molecular chaperone. Structural comparisons between DJ-1, Hsp31, and an Archaea protease, a member of ThiJ/PfpI family, lead to the identification of the chaperone activity of DJ-1 and the proteolytic activity of Hsp31. Moreover, the comparisons provide insights into how the functional diversity is realized in proteins that share an evolutionarily conserved domain. On the basis of the chaperone activity the possible role of DJ-1 in the pathogenesis of Parkinson's disease is discussed.

MeSH Terms
Amino Acid Sequence Binding Sites Chemical Phenomena Chemistry, Physical Computer Simulation Conserved Sequence Crystallization Dimerization Escherichia coli Proteins/chemistry,genetics,metabolism Gene Deletion Humans Intracellular Signaling Peptides and Proteins Luciferases/metabolism Models, Molecular Molecular Chaperones/chemistry,genetics,metabolism Molecular Sequence Data Molecular Structure Mutagenesis Nerve Tissue Proteins/genetics Oncogene Proteins/chemistry,genetics,metabolism Oxidation-Reduction Parkinson Disease/genetics Peptide Fragments/genetics Peptide Hydrolases/metabolism Point Mutation Protein Deglycase DJ-1 Protein Structure, Quaternary Synucleins Ubiquitin-Protein Ligases/genetics
Chemicals
Escherichia coli Proteins Intracellular Signaling Peptides and Proteins Molecular Chaperones Nerve Tissue Proteins Oncogene Proteins Peptide Fragments Synucleins hchA protein, E coli Luciferases Ubiquitin-Protein Ligases parkin protein PARK7 protein, human Protein Deglycase DJ-1 Peptide Hydrolases
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Lee Sun-Joo
Beamline Division, Pohang Accelerator Laboratory, Pohang, 790-784, Kyungbuk, Republic of Korea.
Kim So Jung
Kim In-Kwon
Ko Junsang
Jeong Chang-Sook
Kim Gyung-Hwa
Park Chankyu
Kang Sa-Ouk
Suh Pann-Ghill
Lee Heung-Soo
Cha Sun-Shin
Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
Published
2003-11-07
Epub
2003-00-25
Pages
44552-9
Language
English
Region
United States
NLM ID
2985121R
Subset
IM
Databases
PDB
Analysis Services
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