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PMID: 12952525 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S. Review

Comparing protein abundance and mRNA expression levels on a genomic scale.

Genome biology ·Vol. 4 ·No. 9 ·2003-00-00 ·Pages 117

Greenbaum D, Colangelo C, Williams K, Gerstein M

Abstract

Attempts to correlate protein abundance with mRNA expression levels have had variable success. We review the results of these comparisons, focusing on yeast. In the process, we survey experimental techniques for determining protein abundance, principally two-dimensional gel electrophoresis and mass-spectrometry. We also merge many of the available yeast protein-abundance datasets, using the resulting larger 'meta-dataset' to find correlations between protein and mRNA expression, both globally and within smaller categories.

MeSH Terms
Animals Electrophoresis, Gel, Two-Dimensional Gene Expression Genomics/methods Humans Mass Spectrometry Proteins/genetics,metabolism Proteomics/methods RNA, Messenger/genetics,metabolism
Chemicals
Proteins RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Greenbaum Dov
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA. [email protected]
Colangelo Christopher
Williams Kenneth
Gerstein Mark
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Article Info
Journal
Genome biology
Abbr.
Genome Biol
ISSN
1474-760X
DOI
10.1186/gb-2003-4-9-117
Email
[email protected]
Published
2003-00-00
Epub
2003-00-29
Pages
117
Language
English
Region
England
NLM ID
100960660
PMCID
PMC193646
Subset
IM
Grants
NHLBI NIH HHS · N01HV28186 · United States
NHLBI NIH HHS · N01-HV-28186 · United States
External Links
PubMed source DOI: 10.1186/gb-2003-4-9-117 PMC Full Text View journal details
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