Home LiteratureArticle Details
PMID: 12954777 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A tissue-specific knockout reveals that Gata1 is not essential for Sertoli cell function in the mouse.

Nucleic acids research ·Vol. 31 ·No. 18 ·2003-09-15 ·Pages 5405-12

Lindeboom F, Gillemans N, Karis A, Jaegle M, Meijer D, Grosveld F, Philipsen S

Abstract

The transcription factor Gata1 is essential for the development of erythroid cells. Consequently, Gata1 null mutants die in utero due to severe anaemia. Outside the haematopoietic system, Gata1 is only expressed in the Sertoli cells of the testis. To elucidate the function of Gata1 in the testis, we made a Sertoli cell-specific knockout of the Gata1 gene in the mouse. We deleted a normally functioning 'floxed' Gata1 gene in pre-Sertoli cells in vivo through the expression of Cre from a transgene driven by the Desert Hedgehog promoter. Surprisingly, Gata1 null testes developed to be morphologically normal, spermatogenesis was not obviously affected and expression levels of putative Gata1 target genes, and other Gata factors, were not altered. We conclude that expression of Gata1 in Sertoli cells is not essential for testis development or spermatogenesis in the mouse.

MeSH Terms
Animals Binding Sites/genetics Cells, Cultured DNA-Binding Proteins/genetics,metabolism Erythroid-Specific DNA-Binding Factors Female Fertility/genetics GATA1 Transcription Factor Gene Expression Gene Expression Regulation, Developmental Integrases/genetics,metabolism Male Mice Mice, Inbred C57BL Mice, Knockout Mice, Transgenic RNA, Messenger/genetics,metabolism Recombination, Genetic Reverse Transcriptase Polymerase Chain Reaction Sertoli Cells/cytology,metabolism Testis/embryology,metabolism Transcription Factors/genetics,metabolism Viral Proteins/genetics,metabolism
Chemicals
DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors GATA1 Transcription Factor Gata1 protein, mouse RNA, Messenger Transcription Factors Viral Proteins Cre recombinase Integrases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lindeboom Fokke
Erasmus MC, Department of Cell Biology, Rotterdam, The Netherlands.
Gillemans Nynke
Karis Alar
Jaegle Martine
Meijer Dies
Grosveld Frank
Philipsen Sjaak
References (47)
47 references, click to expand
  1. A short amino acid sequence able to specify nuclear location.
    Cell. 1984 Dec;39(3 Pt 2):499-509 PMID: 6096007
  2. Gonadal differentiation, sex determination and normal Sry expression in mice require direct interaction between transcription partners GATA4 and FOG2.
    Development. 2002 Oct;129(19):4627-34 PMID: 12223418
  3. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells.
    Nature. 1989 Jun 8;339(6224):446-51 PMID: 2725678
  4. Characterization and regulation of testicular inhibin beta-subunit mRNA.
    Mol Endocrinol. 1989 Jun;3(6):939-48 PMID: 2739657
  5. Regulation of testicular inhibin subunit messenger ribonucleic acid levels in vivo: effects of hypophysectomy and selective follicle-stimulating hormone replacement.
    Endocrinology. 1989 Sep;125(3):1630-7 PMID: 2503372
  6. The erythroid-specific transcription factor Eryf1: a new finger protein.
    Cell. 1989 Sep 8;58(5):877-85 PMID: 2776214
  7. Expression of an erythroid transcription factor in megakaryocytic and mast cell lineages.
    Nature. 1990 Mar 29;344(6265):444-7 PMID: 2320112
  8. Megakaryocytic and erythrocytic lineages share specific transcription factors.
    Nature. 1990 Mar 29;344(6265):447-9 PMID: 2320113
  9. Abnormal sexual development in transgenic mice chronically expressing müllerian inhibiting substance.
    Nature. 1990 May 10;345(6271):167-70 PMID: 2336108
  10. Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1.
    Nature. 1991 Jan 17;349(6306):257-60 PMID: 1987478
  11. Interaction of the erythroid transcription factor cGATA-1 with a critical auto-regulatory element.
    Nucleic Acids Res. 1992 Sep 11;20(17):4429-36 PMID: 1408744
  12. Erythroid transcription factor GATA-1 is abundantly transcribed in mouse testis.
    Nature. 1993 Apr 1;362(6419):466-8 PMID: 8464479
  13. Expression of mRNA for the GATA-binding proteins in human eosinophils and basophils: potential role in gene transcription.
    Blood. 1993 Jun 15;81(12):3234-41 PMID: 8507862
  14. Dicistronic targeting constructs: reporters and modifiers of mammalian gene expression.
    Proc Natl Acad Sci U S A. 1994 May 10;91(10):4303-7 PMID: 8183905
  15. Developmental stage- and spermatogenic cycle-specific expression of transcription factor GATA-1 in mouse Sertoli cells.
    Development. 1994 Jul;120(7):1759-66 PMID: 7924983
  16. Novel insights into erythroid development revealed through in vitro differentiation of GATA-1 embryonic stem cells.
    Genes Dev. 1994 May 15;8(10):1184-97 PMID: 7926723
  17. Negative control of the rat inhibin alpha subunit promoter in MA-10 Leydig tumour cells.
    J Mol Endocrinol. 1994 Aug;13(1):39-47 PMID: 7999254
  18. GATA transcription factors: key regulators of hematopoiesis.
    Exp Hematol. 1995 Feb;23(2):99-107 PMID: 7828675
  19. Negative regulation of chicken GATA-1 promoter activity mediated by a hormone response element.
    Mol Endocrinol. 1995 Sep;9(9):1135-46 PMID: 7491106
  20. Sertoli cell signaling by Desert hedgehog regulates the male germline.
    Curr Biol. 1996 Mar 1;6(3):298-304 PMID: 8805249
  21. Multiplex Cre/lox recombination permits selective site-specific DNA targeting to both a natural and an engineered site in the yeast genome.
    Nucleic Acids Res. 1996 Dec 1;24(23):4608-13 PMID: 8972844
  22. The human beta-globin locus control region confers an early embryonic erythroid-specific expression pattern to a basic promoter driving the bacterial lacZ gene.
    Development. 1996 Dec;122(12):3991-9 PMID: 9012519
  23. Conserved structure, regulatory elements, and transcriptional regulation from the GATA-1 gene testis promoter.
    J Biochem. 1997 Feb;121(2):251-63 PMID: 9089398
  24. GATA-1 transcription is controlled by distinct regulatory mechanisms during primitive and definitive erythropoiesis.
    Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4487-92 PMID: 9114016
  25. A lineage-selective knockout establishes the critical role of transcription factor GATA-1 in megakaryocyte growth and platelet development.
    EMBO J. 1997 Jul 1;16(13):3965-73 PMID: 9233806
  26. Testicular GATA-1 factor up-regulates the promoter activity of rat inhibin alpha-subunit gene in MA-10 Leydig tumor cells.
    Mol Endocrinol. 1998 Mar;12(3):378-90 PMID: 9514155
  27. Transcription factor GATA-4 is expressed in a sexually dimorphic pattern during mouse gonadal development and is a potent activator of the Müllerian inhibiting substance promoter.
    Development. 1998 Jul;125(14):2665-75 PMID: 9636081
  28. Suppression of GATA factor activity causes axis duplication in Xenopus.
    Development. 1998 Dec;125(23):4595-605 PMID: 9806909
  29. Generalized lacZ expression with the ROSA26 Cre reporter strain.
    Nat Genet. 1999 Jan;21(1):70-1 PMID: 9916792
  30. Expression and regulation of transcription factors GATA-4 and GATA-6 in developing mouse testis.
    Endocrinology. 1999 Mar;140(3):1470-80 PMID: 10067876
  31. Different sequence requirements for expression in erythroid and megakaryocytic cells within a regulatory element upstream of the GATA-1 gene.
    Development. 1999 Jun;126(12):2799-811 PMID: 10331989
  32. CCAAT enhancer-binding protein beta and GATA-4 binding regions within the promoter of the steroidogenic acute regulatory protein (StAR) gene are required for transcription in rat ovarian cells.
    J Biol Chem. 1999 Jun 18;274(25):17987-96 PMID: 10364248
  33. GATA-6 activates transcription of surfactant protein A.
    J Biol Chem. 2000 Jan 14;275(2):1043-9 PMID: 10625644
  34. Endogenous expression of Müllerian inhibiting substance in early postnatal rat sertoli cells requires multiple steroidogenic factor-1 and GATA-4-binding sites.
    Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1624-9 PMID: 10677509
  35. Familial dyserythropoietic anaemia and thrombocytopenia due to an inherited mutation in GATA1.
    Nat Genet. 2000 Mar;24(3):266-70 PMID: 10700180
  36. GATA-1 is a potential repressor of anti-Müllerian hormone expression during the establishment of puberty in the mouse.
    Mol Reprod Dev. 2000 Jun;56(2):124-38 PMID: 10813843
  37. GATA factor transgenes under GATA-1 locus control rescue germline GATA-1 mutant deficiencies.
    Blood. 2000 Aug 1;96(3):910-6 PMID: 10910904
  38. Characterization of a novel silencer element in the human aromatase gene PII promoter.
    Breast Cancer Res Treat. 2000 Jul;62(2):151-9 PMID: 11016753
  39. GATA-1 and GATA-4 transactivate inhibin/activin beta-B-subunit gene transcription in testicular cells.
    Mol Endocrinol. 2000 Nov;14(11):1820-35 PMID: 11075815
  40. The zinc finger-containing transcription factors GATA-4, -5, and -6. Ubiquitously expressed regulators of tissue-specific gene expression.
    J Biol Chem. 2000 Dec 15;275(50):38949-52 PMID: 11042222
  41. Editorial: gonadal-specific transcription factors--gata (go) 4 it!
    Endocrinology. 2001 Mar;142(3):974-6 PMID: 11181508
  42. GATA factors differentially activate multiple gonadal promoters through conserved GATA regulatory elements.
    Endocrinology. 2001 Mar;142(3):977-86 PMID: 11181509
  43. Platelet characteristics in patients with X-linked macrothrombocytopenia because of a novel GATA1 mutation.
    Blood. 2001 Jul 1;98(1):85-92 PMID: 11418466
  44. E2F and GATA-1 are required for the Sertoli cell-specific promoter activity of the follicle-stimulating hormone receptor gene.
    J Androl. 2001 Jul-Aug;22(4):629-39 PMID: 11451360
  45. The transcription factors GATA4 and GATA6 regulate cardiomyocyte hypertrophy in vitro and in vivo.
    J Biol Chem. 2001 Aug 10;276(32):30245-53 PMID: 11356841
  46. X-linked thrombocytopenia caused by a novel mutation of GATA-1.
    Blood. 2001 Nov 1;98(9):2681-8 PMID: 11675338
  47. Expression and regulation of testicular inhibin alpha-subunit gene in vivo and in vitro.
    Mol Endocrinol. 1989 Jan;3(1):29-35 PMID: 2492634
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkg723
Published
2003-09-15
Pages
5405-12
Language
English
Region
England
NLM ID
0411011
PMCID
PMC203309
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkg723 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2