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PMID: 12963708 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nup358 integrates nuclear envelope breakdown with kinetochore assembly.

The Journal of cell biology ·Vol. 162 ·No. 6 ·2003-09-15 ·Pages 991-1001

Salina D, Enarson P, Rattner JB, Burke B

Abstract

Nuclear envelope breakdown (NEBD) and release of condensed chromosomes into the cytoplasm are key events in the early stages of mitosis in metazoans. NEBD involves the disassembly of all major structural elements of the nuclear envelope, including nuclear pore complexes (NPCs), and the dispersal of nuclear membrane components. The breakdown process is facilitated by microtubules of the mitotic spindle. After NEBD, engagement of spindle microtubules with chromosome-associated kinetochores leads to chromatid segregation. Several NPC subunits relocate to kinetochores after NEBD. siRNA-mediated depletion of one of these proteins, Nup358, reveals that it is essential for kinetochore function. In the absence of Nup358, chromosome congression and segregation are severely perturbed. At the same time, the assembly of other kinetochore components is strongly inhibited, leading to aberrant kinetochore structure. The implication is that Nup358 plays an essential role in integrating NEBD with kinetochore maturation and function. Mitotic arrest associated with Nup358 depletion further suggests that mitotic checkpoint complexes may remain active at nonkinetochore sites.

MeSH Terms
Chromosome Segregation/genetics Eukaryotic Cells/metabolism,ultrastructure Fluorescent Antibody Technique Genes, cdc/physiology HeLa Cells Humans Kinetochores/metabolism,ultrastructure Microscopy, Electron Mitosis/physiology Molecular Chaperones Nuclear Envelope/genetics,metabolism Nuclear Pore Complex Proteins/antagonists & inhibitors,genetics,metabolism RNA Interference Spindle Apparatus/genetics,metabolism
Chemicals
Molecular Chaperones Nuclear Pore Complex Proteins ran-binding protein 2
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Salina Davide
Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610-0235, USA.
Enarson Paul
Rattner J B
Burke Brian
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200304080
Published
2003-09-15
Epub
2003-00-08
Pages
991-1001
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2172838
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200304080 PMC Full Text View journal details
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