A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification.

Research in microbiology ·Vol. 143 ·No. 8 ·1992-10-00 ·Pages 785-90

de Lamballerie X, Zandotti C, Vignoli C, Bollet C, de Micco P

Abstract

"Chelex 100" chelating resin has been previously proposed for the rapid extraction of human DNA for polymerase chain reaction. Protocols are given for the rapid extraction of bacterial and viral DNA from cultures or clinical samples. The DNA samples obtained were suitable for use in polymerase chain reaction.

MeSH Terms

Chelating Agents DNA, Bacterial/isolation & purification Gene Amplification Gram-Negative Bacteria/genetics Gram-Positive Bacteria/genetics In Vitro Techniques Polymerase Chain Reaction/methods Resins, Synthetic Streptococcus agalactiae/genetics

Chemicals

Chelating Agents DNA, Bacterial Resins, Synthetic Chelex 100

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

de Lamballerie X

Laboratoire de Microbiologie et d'Hygiène Hospitalières, Hôpital Salvator, Marseille, France.

Zandotti C

Vignoli C

Bollet C

de Micco P

Article Info

Journal

Research in microbiology

Abbr.

Res Microbiol

ISSN

0923-2508

DOI

10.1016/0923-2508(92)90107-y

Published

1992-10-00

Pages

785-90

Language

English

Region

France

NLM ID

8907468

Subset

External Links

PubMed source DOI: 10.1016/0923-2508(92)90107-y View journal details

Analysis Services

A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification.

Contact

WeChat Official Account