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PMID: 1309938 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mapping T-cell receptor-peptide contacts by variant peptide immunization of single-chain transgenics.

Nature ·Vol. 355 ·No. 6357 ·1992-01-16 ·Pages 224-30

Jorgensen JL, Esser U, Fazekas de St Groth B, Reay PA, Davis MM

Abstract

To test models of T-cell recognition, mice transgenic for T-cell receptor alpha or beta chain have been immunized with variant peptides that force changes in the resulting T-cell response. In particular, charge substitutions on the peptide often elicit reciprocal charges in the junctional (CDR3) sequences of T-cell receptor V alpha or V beta chains, indicating direct T-cell receptor-peptide contact, and allowing derivation of a topology for the T-cell receptor-MHC interaction. At one position on the peptide, variants transformed a homogeneous V beta response into a very heterogeneous one.

MeSH Terms

Amino Acid Sequence Animals Antigens, Differentiation, T-Lymphocyte/genetics,immunology Base Sequence Binding, Competitive CD3 Complex Columbidae Cytochrome c Group/immunology,metabolism Genetic Variation Kinetics Lymph Nodes/immunology Lymphocyte Activation Lymphocytes/immunology Macromolecular Substances Major Histocompatibility Complex Mice Mice, Transgenic Models, Structural Molecular Sequence Data Moths Oligodeoxyribonucleotides Protein Conformation Receptors, Antigen, T-Cell/genetics,immunology,metabolism

Chemicals

Antigens, Differentiation, T-Lymphocyte CD3 Complex Cytochrome c Group Macromolecular Substances Oligodeoxyribonucleotides Receptors, Antigen, T-Cell

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Jorgensen J L

Department of Microbiology and Immunology, Stanford University, California 94305.

Esser U

Fazekas de St Groth B

Reay P A

Davis M M

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/355224a0

Published

1992-01-16

Pages

224-30

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/355224a0 View journal details

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Mapping T-cell receptor-peptide contacts by variant peptide immunization of single-chain transgenics.

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