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PMID: 1311719 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Platelet-derived growth factor receptors on macrovascular endothelial cells mediate relaxation via nitric oxide in rat aorta.

The Journal of clinical investigation ·Vol. 89 ·No. 3 ·1992-03-00 ·Pages 878-82

Cunningham LD, Brecher P, Cohen RA

Abstract

The effects of platelet-derived growth factor (PDGF) were studied in isolated rings of rat aorta contracted submaximally to phenylephrine. The BB isoform of PDGF elicited relaxation in rings with endothelium and further contraction in rings without endothelium. Both the endothelium-dependent relaxation and endothelium-independent contraction occurred at concentrations known to induce PDGF receptor-mediated responses in cultured cells. Furthermore, the relaxation was isoform specific. This conclusion is supported by the unique ability of PDGF-BB to induce endothelium-dependent relaxations, as well as by studies showing isoform specific, concentration-dependent desensitization of PDGF-BB relaxation. The relaxation induced by PDGF-BB was prevented by N omega-nitro-L-arginine. It was also observed that endothelium-independent contractions to the AB and AA isoforms of PDGF were less than those to PDGF-BB. Contrary to the widely held view that PDGF receptors are not present on the endothelium of macrovessels, these studies provide evidence for an endothelium-dependent, nitric oxide mediated relaxation of rat aorta caused by PDGF via PDGF beta beta-receptors.

MeSH Terms
Animals Aorta/physiology Endothelium, Vascular/physiology In Vitro Techniques Male Nitric Oxide/metabolism Platelet-Derived Growth Factor/pharmacology Rats Receptors, Cell Surface/physiology Receptors, Platelet-Derived Growth Factor Vasodilation/drug effects
Chemicals
Platelet-Derived Growth Factor Receptors, Cell Surface Nitric Oxide Receptors, Platelet-Derived Growth Factor
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cunningham L D
Vascular Biology Unit, Evans Memorial Department of Clinical Research, Boston, Massachusetts.
Brecher P
Cohen R A
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI115667
Published
1992-03-00
Pages
878-82
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC442933
Subset
IM
Grants
NHLBI NIH HHS · HL-31607 · United States
NHLBI NIH HHS · HL-38731 · United States
NHLBI NIH HHS · HL-47124 · United States
External Links
PubMed source DOI: 10.1172/JCI115667 PMC Full Text View journal details
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