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PMID: 1312080 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Reduced levels of transforming growth factor-beta type I receptor in human gastric carcinomas.

Japanese journal of cancer research : Gann ·Vol. 83 ·No. 1 ·1992-01-00 ·Pages 86-92

Ito M, Yasui W, Nakayama H, Yokozaki H, Ito H, Tahara E

Abstract

The expressions of transforming growth factor beta (TGF-beta) and its receptor and TGF-beta inhibitory element (TIE)-binding protein were examined on human gastric carcinomas by Northern blot hybridization, immunohistochemistry, affinity labeling and gel retardation analysis. TGF-beta mRNA was expressed in tumor and normal tissues at various levels. Immunohistochemically, TGF-beta expression was confirmed to be present within tumor cells. Out of the 17 human gastric carcinoma tissues, 14 (82%) showed a reduction in the level of type I receptor (65 kDa) for TGF-beta when compared to corresponding normal mucosas. Interestingly, in seven of the 14 tumors the level of TIE-binding protein in the tumor tissue was lower than that in normal mucosa. Human gastric carcinoma cell line TMK-1, whose growth was inhibited by TGF-beta, had only type I receptor for TGF-beta and showed a high level of TIE-binding protein. Conversely, MKN-1, a TGF-beta-resistant cell line, exhibited an extremely low level of TGF-beta receptor and had no TIE-binding protein. These results overall indicate that although human gastric carcinoma cells produced TGF-beta, they showed a reduction in TGF-beta type I receptor and a low level of TIE-binding protein, resulting in escape from growth inhibition by TGF-beta.

MeSH Terms
Aged Carrier Proteins/metabolism Cell Line/metabolism DNA Replication Female Humans Intracellular Signaling Peptides and Proteins Latent TGF-beta Binding Proteins Male Middle Aged Receptors, Cell Surface/analysis Receptors, Transforming Growth Factor beta Stomach Neoplasms/metabolism Transforming Growth Factor beta/analysis
Chemicals
Carrier Proteins Intracellular Signaling Peptides and Proteins Latent TGF-beta Binding Proteins Receptors, Cell Surface Receptors, Transforming Growth Factor beta Transforming Growth Factor beta
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ito M
First Department of Pathology, Hiroshima University School of Medicine.
Yasui W
Nakayama H
Yokozaki H
Ito H
Tahara E
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Article Info
Journal
Japanese journal of cancer research : Gann
Abbr.
Jpn J Cancer Res
ISSN
0910-5050
DOI
10.1111/j.1349-7006.1992.tb02356.x
Published
1992-01-00
Pages
86-92
Language
English
Region
Japan
NLM ID
8509412
PMCID
PMC5918645
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1349-7006.1992.tb02356.x PMC Full Text
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