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PMID: 1314809 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The low-affinity component of Saccharomyces cerevisiae maltose transport is an artifact.

Journal of bacteriology ·Vol. 174 ·No. 9 ·1992-05-00 ·Pages 3065-9

Benito B, Lagunas R

Abstract

It has been reported by several laboratories that maltose transport in Saccharomyces cerevisiae consists of two components with high- and low-affinity constants for maltose. We have investigated the characteristics of the low-affinity component and have found that it shows an abnormal behavior without similarity to any transport mechanism described in this organism. The results strongly indicate that this apparent transport activity is due not to a genuine transport process but to nonspecific binding of maltose to the cell wall and plasma membrane.

MeSH Terms
Biological Transport Cell Division Cell Membrane/metabolism Cell Wall/metabolism Glucose/metabolism Isotope Labeling Kinetics Maltose/metabolism Protons Saccharomyces cerevisiae/metabolism
Chemicals
Protons Maltose Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Benito B
Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Lagunas R
References (14)
14 references, click to expand
  1. Energy requirements for maltose transport in yeast.
    Eur J Biochem. 1977 Oct 17;80(1):97-102 PMID: 21792
  2. Increased endocytosis in the Saccharomyces cerevisiae fragile mutant VY1160.
    Yeast. 1991 Apr;7(3):211-7 PMID: 1882547
  3. MAL11 and MAL61 encode the inducible high-affinity maltose transporter of Saccharomyces cerevisiae.
    J Bacteriol. 1991 Mar;173(5):1817-20 PMID: 1999393
  4. 2-Deoxy-D-glucose resistant yeast with altered sugar transport activity.
    FEBS Lett. 1990 Aug 20;269(1):202-4 PMID: 2201568
  5. Characteristics of galactose transport in Saccharomyces cerevisiae cells and reconstituted lipid vesicles.
    J Bacteriol. 1989 Jun;171(6):3539-44 PMID: 2656659
  6. Catabolite inactivation of the glucose transport system in Saccharomyces cerevisiae.
    J Gen Microbiol. 1986 Feb;132(2):379-85 PMID: 3519857
  7. On the evaluation of the constants Vm and KM in enzyme reactions.
    Science. 1952 Sep 26;116(3013):329-31 PMID: 12984118
  8. Relationship between sugar structure and competition for the sugar transport system in Bakers' yeast.
    J Bacteriol. 1968 Feb;95(2):603-11 PMID: 5640385
  9. Specificity of the constitutive hexose transport in yeast.
    Eur J Biochem. 1968 Aug;5(3):321-9 PMID: 5680352
  10. Purification and characterization of maltase and alpha-methyl glucosidase from yeast.
    Biochim Biophys Acta. 1967 Sep 12;146(1):173-80 PMID: 6060462
  11. Transport of maltose in Saccharomyces cerevisiae. Effect of pH and potassium ions.
    Biochem J. 1984 Sep 1;222(2):293-8 PMID: 6383358
  12. Transport of sugars in yeasts. II. Mechanisms of utilization of disaccharides and related glycosides.
    Biochim Biophys Acta. 1962 Jan 1;56:49-62 PMID: 13884257
  13. Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1730-4 PMID: 6300872
  14. Endocytosis in yeast: several of the yeast secretory mutants are defective in endocytosis.
    Cell. 1985 Apr;40(4):1001-9 PMID: 3886157
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1992-05-00
Pages
3065-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205962
Subset
IM
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