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PMID: 1315040 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Fusogenic segments of bovine leukemia virus and simian immunodeficiency virus are interchangeable and mediate fusion by means of oblique insertion in the lipid bilayer of their target cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 9 ·1992-05-01 ·Pages 3810-4

Vonèche V, Portetelle D, Kettmann R, Willems L, Limbach K, Paoletti E, Ruysschaert JM, Burny A, Brasseur R

Abstract

Modified bovine leukemia virus (BLV) glycoproteins were expressed by using vaccinia virus recombinants, and their fusogenic capacities were examined by a syncytia-formation assay. This analysis indicates that (i) both BLV envelope glycoproteins gp51 and gp30 are necessary for cell fusion; (ii) insertion of the N-terminal segment of gp30 (fusion peptide) into the lipid bilayer in an oblique orientation, as predicted by computer conformational analysis, results in fusogenic capacities higher than insertion in a perpendicular or parallel orientation; and (iii) replacement of the BLV fusion peptide with its simian immunodeficiency virus counterpart does not modify the fusogenic capacity of the BLV glycoprotein.

MeSH Terms
Amino Acid Sequence Cell Fusion Cell Membrane/ultrastructure Cloning, Molecular DNA Mutational Analysis Leukemia Virus, Bovine/physiology Lipid Bilayers Membrane Glycoproteins/physiology Molecular Sequence Data Protein Conformation Simian Immunodeficiency Virus/physiology Structure-Activity Relationship Viral Fusion Proteins/immunology,physiology
Chemicals
Lipid Bilayers Membrane Glycoproteins Viral Fusion Proteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Vonèche V
Faculty of Agronomy, Gembloux, Belgium.
Portetelle D
Kettmann R
Willems L
Limbach K
Paoletti E
Ruysschaert J M
Burny A
Brasseur R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.9.3810
Published
1992-05-01
Pages
3810-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC525580
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.9.3810 PMC Full Text View journal details
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