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PMID: 1315317 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Neutrophil migration across monolayers of cytokine-prestimulated endothelial cells: a role for platelet-activating factor and IL-8.

The Journal of cell biology ·Vol. 117 ·No. 3 ·1992-05-00 ·Pages 565-72

Kuijpers TW, Hakkert BC, Hart MH, Roos D

Abstract

In a previous study we observed that neutrophils respond with a rapid rise in [Ca2+]i during adherence to cytokine-activated endothelial cells (EC), caused by EC membrane-associated platelet-activating factor (PAF). In the present study, we investigated whether this form of PAF was important in neutrophil adherence and migration across monolayers of rIL-1 beta- or rTNF alpha-prestimulated EC. PAF receptor antagonists prevented neutrophil migration across cytokine-pretreated EC by approximately 60% (P less than 0.005) without interfering with the process of adherence. The antagonists WEB 2086 and L-652,731 had no effect on neutrophil migration across resting EC induced by formylmethionyl-leucyl-phenylalanine (FMLP). A murine anti-IL-8 antiserum was found to also partially inhibit the neutrophil transmigration across cytokine-activated EC. When the anti-IL-8 antiserum was used in combination with a PAF receptor antagonist, neutrophil migration across cytokine-pretreated monolayers of EC was completely prevented. During transmigration, LAM-1 and CD44 on the neutrophils were down-modulated; both WEB 2086 and anti-IL-8 antiserum partially prevented this down-modulation caused by cytokine-prestimulated EC. Our results indicate that human neutrophils are activated and guided by EC-associated PAF and EC-derived IL-8 during the in vitro diapedesis in between cytokine-stimulated EC.

MeSH Terms
Antigens, Surface/biosynthesis Azepines/pharmacology Cell Adhesion/physiology Cell Movement/physiology Cells, Cultured Endothelium, Vascular/drug effects,growth & development,physiology Female Furans/pharmacology Humans Interleukin-8/pharmacology Neutrophils/physiology Platelet Activating Factor/antagonists & inhibitors,metabolism Platelet Membrane Glycoproteins Pregnancy Receptors, Cell Surface/antagonists & inhibitors,physiology Receptors, G-Protein-Coupled Stimulation, Chemical Triazoles/pharmacology Tumor Necrosis Factor-alpha/pharmacology Umbilical Veins
Chemicals
Antigens, Surface Azepines Furans Interleukin-8 Platelet Activating Factor Platelet Membrane Glycoproteins Receptors, Cell Surface Receptors, G-Protein-Coupled Triazoles Tumor Necrosis Factor-alpha platelet activating factor receptor WEB 2086 2,5-bis(3,4,5-trimethoxyphenyl)tetrahydrofuran
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kuijpers T W
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Hakkert B C
Hart M H
Roos D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.117.3.565
Published
1992-05-00
Pages
565-72
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289445
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.117.3.565 PMC Full Text View journal details
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