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PMID: 1315782 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A soluble form of the F3 neuronal cell adhesion molecule promotes neurite outgrowth.

The Journal of cell biology ·Vol. 117 ·No. 4 ·1992-05-00 ·Pages 877-87

Durbec P, Gennarini G, Goridis C, Rougon G

Abstract

The F3 molecule is a member of the immunoglobulin superfamily anchored to membranes by a glycane-phosphatidylinositol, and is predominantly expressed on subsets of axons of the central and peripheral nervous system. In a previous paper (Gennarini, G., P. Durbec, A. Boned, G. Rougon, and C. Goridis. 1991. Neuron. 6:595-606), we have established that F3 fulfills the operational definition of a cell adhesion molecule and that it stimulates neurite outgrowth when presented to sensory neurons as a surface component of transfected CHO cells. In the present study the question as to whether soluble forms of F3 would be functionally active was addressed in vitro on cultures of mouse dorsal root ganglion neurons. We observed that preparations enriched in soluble F3 had no effect on neuron attachment but enhanced neurite initiation and neurite outgrowth in a dose-dependent manner. By contrast, soluble NCAM-120 does not have any measurable effect on these phenomena. Addition of anti-F3 monovalent antibodies reduced the number of process-bearing neurons and the neuritic output per neuron to control values. Addition of cerebrospinal fluid, a natural source of soluble F3, also stimulated neurite extension, and this effect was partially blocked by anti-F3 antibodies. Our results suggest that the soluble forms of adhesive proteins with neurite outgrowth-promoting properties could act at a distance from their site of release in a way reminiscent of growth and trophic factors.

MeSH Terms
Animals Cell Adhesion Molecules/chemistry,physiology Cell Adhesion Molecules, Neuronal/chemistry,physiology Cell Survival Cerebrospinal Fluid/physiology Contactins Ganglia, Spinal Laminin/metabolism Mice Nerve Tissue Proteins/chemistry,physiology Neurites/ultrastructure Neurons/cytology Phosphatidylinositol Diacylglycerol-Lyase Phosphoric Diester Hydrolases/metabolism Solubility
Chemicals
Cell Adhesion Molecules Cell Adhesion Molecules, Neuronal Contactins Laminin Nerve Tissue Proteins Phosphoric Diester Hydrolases Phosphatidylinositol Diacylglycerol-Lyase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Durbec P
Biologie de la Différenciation Cellulaire, CNRS URA 179, Marseille, France.
Gennarini G
Goridis C
Rougon G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.117.4.877
Published
1992-05-00
Pages
877-87
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289471
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.117.4.877 PMC Full Text View journal details
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