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PMID: 1316274 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Casein kinase II phosphorylates the eukaryote-specific C-terminal domain of topoisomerase II in vivo.

The EMBO journal ·Vol. 11 ·No. 5 ·1992-05-00 ·Pages 1785-96

Cardenas ME, Dang Q, Glover CV, Gasser SM

Abstract

The decatenation activity of DNA topoisomerase II is essential for viability as eukaryotic cells traverse mitosis. Phosphorylation has been shown to stimulate topoisomerase II activity in vitro. Here we show that topoisomerase II is a phosphoprotein in yeast and that the level of incorporated phosphate is significantly higher at mitosis than in G1. Comparison of tryptic phosphopeptide maps reveals that the major phosphorylation sites in vivo are targets for casein kinase II. Incorporation of phosphate into topoisomerase II is nearly undetectable at the non-permissive temperature in a conditional casein kinase II mutant. The sites modified by casein kinase II are located in the extreme C-terminal domain of topoisomerase II. This domain is absent in prokaryotic and highly divergent among eukaryotic type II topoisomerases, and may serve to regulate functions of topoisomerase II that are unique to eukaryotic cells.

MeSH Terms
Amino Acids/analysis Casein Kinase II Cell Cycle DNA Topoisomerases, Type II/genetics,metabolism Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Peptide Mapping Phosphorylation Precipitin Tests Protein Serine-Threonine Kinases/metabolism Saccharomyces cerevisiae/cytology,enzymology
Chemicals
Amino Acids Casein Kinase II Protein Serine-Threonine Kinases DNA Topoisomerases, Type II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cardenas M E
Swiss Institute for Experimental Cancer Research, Epalinges s/Lausanne.
Dang Q
Glover C V
Gasser S M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-05-00
Pages
1785-96
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556636
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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