Packaging system for rapid production of murine leukemia virus vectors with variable tropism.
A method for rapidly producing helper-free murine leukemia virus (MLV) without using packaging cell lines is described. Viruses bearing ecotropic or amphotropic MLV or Rous sarcoma virus envelope glycoprotein and containing various retroviral vector genomes have been prepared with titers 30 to 40-fold higher than those produced by transient transfection of standard packaging cells. This system can be used to alter the cellular tropism of MLV by incorporating other envelope glycoproteins and to prepare retroviral vector stocks without establishing stable producer cell lines. This method will be particularly useful for preparing viruses that encode toxic proteins and for the rapid analysis of panels of mutant envelope glycoproteins.
12 references, click to expand
-
Gene expression from both intronless and intron-containing Rous sarcoma virus clones is specifically inhibited by anti-sense RNA.Mol Cell Biol. 1985 Sep;5(9):2341-8 PMID: 2426579
-
Construction and characterization of a retroviral vector demonstrating efficient expression of cloned cDNA sequences.DNA. 1988 Apr;7(3):219-25 PMID: 2836147
-
Expression of human class II major histocompatibility complex antigens using retrovirus vectors.Proc Natl Acad Sci U S A. 1987 Apr;84(8):2150-4 PMID: 3031667
-
Development of retrovirus vectors useful for expressing genes in cultured murine embryonal cells and hematopoietic cells in vivo.J Virol. 1988 Oct;62(10):3795-801 PMID: 3418785
-
Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.Cell. 1983 May;33(1):153-9 PMID: 6678608
-
Interaction of the unique N-terminal region of tyrosine kinase p56lck with cytoplasmic domains of CD4 and CD8 is mediated by cysteine motifs.Cell. 1990 Mar 9;60(5):755-65 PMID: 2107025
-
BCR first exon sequences specifically activate the BCR/ABL tyrosine kinase oncogene of Philadelphia chromosome-positive human leukemias.Mol Cell Biol. 1991 Apr;11(4):1785-92 PMID: 2005881
-
Human cells infected with retrovirus vectors acquire an endogenous murine provirus.J Virol. 1990 Jan;64(1):424-7 PMID: 2152828
-
Structure and expression of the herpes simplex virus type 2 glycoprotein gB gene.J Virol. 1987 Feb;61(2):326-35 PMID: 3027364
-
Lack of competition results in efficient packaging of heterologous murine retroviral RNAs and reticuloendotheliosis virus encapsidation-minus RNAs by the reticuloendotheliosis virus helper cell line.J Virol. 1987 Sep;61(9):2675-83 PMID: 3039161
-
Pseudotyping with human T-cell leukemia virus type I broadens the human immunodeficiency virus host range.J Virol. 1991 Jan;65(1):162-9 PMID: 1845882
-
Naturally occurring murine leukemia viruses in wild mice: characterization of a new "amphotropic" class.J Virol. 1976 Jul;19(1):19-25 PMID: 59816
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
