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PMID: 1321834 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Simple in vitro methods for titrating feline immunodeficiency virus (FIV) and FIV neutralizing antibodies.

Journal of virological methods ·Vol. 37 ·No. 3 ·1992-06-00 ·Pages 241-52

Tozzini F, Matteucci D, Bandecchi P, Baldinotti F, Poli A, Pistello M, Siebelink KH, Ceccherini-Nelli L, Bendinelli M

Abstract

The feline immunodeficiency virus (FIV) readily produced syncytia in Crandell feline kidney (CrFK) cells adapted to a medium containing 0.5% fetal calf serum, a variety of growth factors and other supplements. This finding has been exploited to develop simple and sensitive virus titration and neutralization assays. High titre neutralizing antibodies were detected in cats infected naturally and experimentally with FIV, but not in uninfected animals.

MeSH Terms

Animals Antibodies, Viral/blood Blotting, Western Cats Cell Line Disease Models, Animal Enzyme-Linked Immunosorbent Assay Feline Acquired Immunodeficiency Syndrome/diagnosis Giant Cells/microbiology Immunodeficiency Virus, Feline/immunology,isolation & purification,physiology Kidney/cytology Neutralization Tests Sensitivity and Specificity

Chemicals

Antibodies, Viral

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Tozzini F

Department of Animal Pathology, University of Pisa, Italy.

Matteucci D

Bandecchi P

Baldinotti F

Poli A

Pistello M

Siebelink K H

Ceccherini-Nelli L

Bendinelli M

Article Info

Journal

Journal of virological methods

Abbr.

J Virol Methods

ISSN

0166-0934

DOI

10.1016/0166-0934(92)90026-a

Published

1992-06-00

Pages

241-52

Language

English

Region

Netherlands

NLM ID

8005839

Subset

External Links

PubMed source DOI: 10.1016/0166-0934(92)90026-a View journal details

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Simple in vitro methods for titrating feline immunodeficiency virus (FIV) and FIV neutralizing antibodies.

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