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PMID: 1323117 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Stable association between the bovine papillomavirus E5 transforming protein and activated platelet-derived growth factor receptor in transformed mouse cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 15 ·1992-08-01 ·Pages 6736-40

Petti L, DiMaio D

Abstract

The 44-amino acid E5 transforming protein of bovine papillomavirus is the shortest protein known to induce tumorigenic transformation of fibroblasts. We showed previously that expression of the E5 protein activates the cellular beta receptor for platelet-derived growth factor (PDGF) and proposed that the activated receptor transmits the transforming signal to the cell. Here we use coimmunoprecipitation analysis to show that the E5 protein and the activated PDGF receptor exist in a stable complex in transformed mouse C127 cells. These results suggest a distinct mechanism of growth factor receptor activation and provide further evidence that the PDGF receptor is an important target of the E5 protein.

MeSH Terms
Animals Bovine papillomavirus 1/genetics,metabolism Cell Line, Transformed Cell Transformation, Viral Electrophoresis, Polyacrylamide Gel Mice Molecular Weight Oncogene Proteins, Viral/genetics,isolation & purification,metabolism Platelet-Derived Growth Factor/metabolism Protein Binding Receptors, Cell Surface/isolation & purification,metabolism Receptors, Platelet-Derived Growth Factor
Chemicals
Oncogene Proteins, Viral Platelet-Derived Growth Factor Receptors, Cell Surface oncogene protein E5, Bovine papillomavirus type 1 Receptors, Platelet-Derived Growth Factor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Petti L
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510.
DiMaio D
References (34)
34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.15.6736
Published
1992-08-01
Pages
6736-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49578
Subset
IM
Grants
NCI NIH HHS · CA37157 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.15.6736 PMC Full Text View journal details
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